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Status |
Public on Jan 06, 2015 |
Title |
Genome-wide identification of Bacillus subtilis Zur-binding sites. |
Organism |
Bacillus subtilis subsp. subtilis str. 168 |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
The transcriptional factor Zur plays a key role in regulating zinc homeostasis in Bacillus subtilis. The genomic sites bound by Zur were mapped using a chromatine immunoprecipitation approach. This allowed the identification of 80 inter- and intragenic chromosomal sites bound by Zur.
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Overall design |
This data set contains 2 samples. Immunoprecipitated (IP) DNA from Bacillus subtilis BSAS36 strain (BSB1, a tryptophan-prototrophic derivative 168 strain expressing a SPA-tagged Zur protein) were extracted from bacterial cells in the exponential growth phase. IP and whole-cell extract DNA were hybridized on tiling array to generate ChIP-on-chip profiles. Two biological replicates were anlyzed.
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Contributor(s) |
Auger S, Prestel E, Noirot P |
Citation(s) |
25649915 |
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Submission date |
Jan 05, 2015 |
Last update date |
Apr 09, 2015 |
Contact name |
Sandrine Auger |
E-mail(s) |
[email protected]
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Phone |
33 1 34 65 25 22
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Organization name |
INRA
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Lab |
MICALIS
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Street address |
INRA-Domaine de Vilvert
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City |
Jouy-en-Josas |
ZIP/Postal code |
F-78350 |
Country |
France |
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Platforms (1) |
GPL13168 |
BaSysBio Bacillus subtilis T2 385K array [090325_CBS_DTU_Bsub_T2] |
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Samples (2) |
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Relations |
BioProject |
PRJNA271606 |