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| Status |
Public on Jul 22, 2010 |
| Title |
Expression data from human liver cell line induced by PCB153 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Exposure to Polychlorobiphenyls (PCBs) is known to cause serious health effects in human but the gene expression profiles leading to development of differnet diseases is not fully understood. The knowledge of global gene expression will help us to devlop early diagnostic biomarkers for PCB induced health effects.
We used microarrays to detail the global gene expression profile underlying the effects of PCB153 on human liver cells leading to identification of distinct classes of up-regulated and down-regulated genes and cellular processes.
Keywords: time course
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| Overall design |
The HepG2 cells were grown for 48 hours in DMEM supplemented with 10% FBS and 1X Pennicillin-Streptromycin and treated with PCB153 for different times. Trizol extraction of total RNA was performed according to the manufacturer's instructions. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microgram of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix). Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HGU133 plus 2.0). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneArray Scanner 3000. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
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| Contributor(s) |
De S, Ghosh S, Moses L, Hoffman EP, Dutta SK |
| Citation(s) |
20638727 |
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| Submission date |
Dec 09, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Sisir K. Dutta |
| E-mail(s) |
[email protected]
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| Phone |
202-806-6942
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| Fax |
202-806-5832
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| Organization name |
Howard University
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| Department |
Biology
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| Lab |
Molecular Genetics
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| Street address |
415 College Street, NW
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| City |
Washington DC |
| State/province |
DC |
| ZIP/Postal code |
20059 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (16)
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| GSM149381 |
PCB153 treated HepG2 cells at T0, biological replicate 1 |
| GSM149382 |
PCB153 treated HepG2 cells at T0, biological replicate 2 |
| GSM149383 |
PCB153 treated HepG2 cells at T0, biological replicate 3 |
| GSM149384 |
PCB153 treated HepG2 cells at T0.5, biological replicate 1 |
| GSM149385 |
PCB153 treated HepG2 cells at T0.5, biological replicate 2 |
| GSM149386 |
PCB153 treated HepG2 cells at T0.5, biological replicate 3 |
| GSM149387 |
PCB153 treated HepG2 cells at T1.5, biological replicate 1 |
| GSM149388 |
PCB153 treated HepG2 cells at T1.5, biological replicate 2 |
| GSM149389 |
PCB153 treated HepG2 cells at T6, biological replicate 1 |
| GSM149390 |
PCB153 treated HepG2 cells at T6, biological replicate 2 |
| GSM149391 |
PCB153 treated HepG2 cells at T18, biological replicate 1 |
| GSM149392 |
PCB153 treated HepG2 cells at T18, biological replicate 2 |
| GSM149393 |
PCB153 treated HepG2 cells at T18, biological replicate 3 |
| GSM154182 |
HepG2 cells at T18 Control, biological replicate 1 |
| GSM154183 |
HepG2 cells at T18 Control, biological replicate 2 |
| GSM154184 |
HepG2 cells at T18 Control, biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE6878 |
Expression data from human liver cells |
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| Relations |
| BioProject |
PRJNA104229 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6494_RAW.tar |
81.7 Mb |
(http)(custom) |
TAR (of CEL) |
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