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| Status |
Public on Jun 22, 2007 |
| Title |
An estrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This study was performed to check that ESR1 and BMI1 are biologically active after lentiviral transduction of primary human mammary epithelial cells (HMECs) with lentiviral vectors expressing ESR1 and BMI1 from the human PGK promoter. ESR1 targets like PGR, PRLR and GREB1, but not TFF1 and XBP1, were induced by estradiol in the ESR1-expressing cells. BMI1 targets like BMI1, NEFL and CCND2 were repressed in the BMI1-expressing cells. BMI1 suppressed genes associated with squamous and neural differentiation in the ESR1 plus BMI1-expressing cells.
Keywords: Drug response
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| Overall design |
Infect with lentiviruses 24 hours after reduction mammmoplasty. Grow cells in human mammosphere medium: Hepes-buffered DMEM/F12 supplemented with 20 ng/ml EGF, 1x B-27 (GIBCO) plus 1 nM 17-β-estradiol or 1 uM fulvestrant (ICI 182,780).
Lentiviral vectors: pSD-82, ESR1; pSD-84, BMI1; pSD-86, gusA.
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| Contributor(s) |
Duss S, André S, Nicoulaz A, Fiche M, Bonnefoi H, Brisken C, Iggo R |
| Citation(s) |
17573968 |
| |
| Submission date |
Dec 16, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Richard Iggo |
| E-mail(s) |
[email protected]
|
| Phone |
+33 5 56 33 04 23
|
| Fax |
+33 5 56 33 32 06
|
| Organization name |
University of Bordeaux
|
| Department |
Bergonie Cancer Institute
|
| Lab |
INSERM U916
|
| Street address |
229 cours de l'Argonne
|
| City |
Bordeaux |
| ZIP/Postal code |
33076 |
| Country |
France |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA98591 |
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