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| Status |
Public on Mar 02, 2007 |
| Title |
High resolution mapping reveals links of HP1 with active and inactive chromatin components |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Heterochromatin protein 1 (HP1) is commonly seen as a key factor of repressive heterochromatin, even though a few genes are known to require HP1-chromatin for their expression. In order to obtain insight into the targeting of HP1 and its interplay with other chromatin components, we have mapped HP1 binding sites on chromosome 2 and 4 in Drosophila Kc cells using high-density oligonucleotide arrays and the DamID technique. The resulting high-resolution maps show that HP1 forms large domains in pericentric regions, but is targeted to single genes on chromosome arms. Intriguingly, HP1 shows a striking preference for exon-dense genes on chromosome arms. Furthermore, HP1 binds along entire transcription units, except for 5’ regions. Comparison with expression data shows that most of these genes are actively transcribed. HP1 target genes are also marked by the histone variant H3.3 and dimethylated histone 3 lysine 4 (H3K4me2), which are both typical of active chromatin. Interestingly, H3.3 deposition, which is usually observed along entire transcription units, is limited to the 5’ ends of HP1-bound genes. Thus, H3.3 and HP1 are mutually exclusive marks on active chromatin. Additionally, we observed that HP1-chromatin and Polycomb-chromatin are non-overlapping, but often closely juxtaposed, suggesting an interplay between both types of chromatin. These results demonstrate that HP1-chromatin is transcriptionally active and has extensive links with several other chromatin components.
Keywords: DamID
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| Overall design |
DamID was performed for the heterochromatin protein HP1. Using high density oligonucleotide arrays we were able to map the binding sites of HP1 with a 100 bp resolution. Three biological replicates were analyzed, with one experiment in the reversed dye orientation.
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| Contributor(s) |
de Wit E, Greil FA, van Steensel B |
| Citation(s) |
17335352 |
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| Submission date |
Dec 19, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Bas van Steensel |
| E-mail(s) |
[email protected]
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| Phone |
+ 31 20 512 2040
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| Fax |
+31 20 669 1383
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| URL |
http://www.nki.nl/nkidep/vansteensel
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| Organization name |
Netherlands Cancer Institute
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| Department |
division of Molecular Biology
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| Lab |
van Steensel group
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| Street address |
Plesmanlaan 121
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| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL2678 |
Tiling design for D. melanogaster |
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| Samples (3) |
| GSM151831 |
Embryonic Drosophila cells, Kc167 cells, High resolution HP1 DamID, replicate 1 |
| GSM151832 |
Embryonic Drosophila cells, Kc167 cells, High resolution HP1 DamID, replicate 2 |
| GSM151833 |
Embryonic Drosophila cells, Kc167 cells, High resolution HP1 DamID, replicate 3 |
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| Relations |
| BioProject |
PRJNA98735 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6564_RAW.tar |
4.0 Mb |
(http)(custom) |
TAR |
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