 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 18, 2015 |
| Title |
Complete genome sequence of Vibrio parahaemolyticus CHN25 and transcriptomic analysis of cold shock proteins of VpaCspA and VpaCspD in the adaptation of low temperature growth |
| Organism |
Vibrio parahaemolyticus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate low-temperature tolerance of the bacterium, three in-frame gene deletion mutants of VpacspA and VpacspD were constructed using homologous recombination method. When compared to the wild type strain, the growth of ΔVpacspA mutant was strongly repressed at 10 0C, whereas the deletion of VpacspD gene greatly activated the bacterium growth at the low temperature. Transcriptome data revealed that 12.4% of the expressed genes in V. parahaemolyticus CHN25 was significantly changed in ΔVpacspA mutant grown at 10 0C, including those involved in amino acid degradation, ATP-binding cassette (ABC) transporters, secretion systems, sulfur and glycerophospholipid metabolisms, whereas the low temperature elicited 10.0% of the genes from ΔVpacspD mutant, such as phosphotransferase system, nitrogen and amino acid metabolisms. Moreover, the major changed metabolic pathways in dual-gene deletion mutant (ΔVpacspAD) differed radically from those in single-gene mutants. Comparison of transcriptome profiles further revealed a number of differentially expressed genes shared among the three mutants, as well as regulators specifically, coordinately and or antagonistically regulating in the adaptation of V. parahaemolyticus CHN25 to the low-temperature growth.
|
| |
|
| Overall design |
mRNA profiles of mid-log phase WT(G), ΔVpacspA, ΔVpacspD and ΔVpacspAD at 10 °C were generated by deep sequencing using Illumina HiSeq 2500
|
| |
|
| Contributor(s) |
Chen L, Zhu C, Sun B |
| Citation(s) |
28583064 |
| |
| Submission date |
Feb 17, 2015 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Lanming Chen |
| E-mail(s) |
[email protected]
|
| Phone |
+86 021 61900504
|
| Organization name |
Shanghai Ocean University
|
| Department |
College of Food Science and Technology
|
| Lab |
Bacteria Genomics and Proteomics
|
| Street address |
999 Hu Cheng Huan Road
|
| City |
Shanghai |
| ZIP/Postal code |
201306 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL19796 |
Illumina HiSeq 2500 (Vibrio parahaemolyticus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA275659 |
| SRA |
SRP055104 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65998_RAW.tar |
640.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...