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| Status |
Public on Feb 01, 2019 |
| Title |
Transcript profiling of Pinus sylvestris trees as a response to Heterobasidion annosum infection under field conditions |
| Organism |
Pinus sylvestris |
| Experiment type |
Expression profiling by array
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| Summary |
In conifer forests of Northern Europe, a pathogenic fungus Heterobasidion annosum attacks the roots of Scots pine and causes mortality. Trees with infection grow slower and produce less timber with reduced quality. Despite applied control methods, such as switching tree species to a non-host species, or stump treatment, root and butt rot continues to be a serious forest health problem. Disease resistance breeding is a less-applied control method which has potential to improve tree health. However, neither conifer genotypes with absolute resistance to Heterobasidion sp. nor robust selection markers for resistance breeding have been found. We studied the responses of various Scots pine genotypes to Heterobasidion annosum infection and mechanic damage in drained peatland. Stems and roots of mature naturally regenerated Scots pine trees growing in drained peatland were either artificially infected with H. annosum or wounded and inoculated with sterile inoculum. Untreated trees from the study sites served as controls. Responses of different Scots pine genotypes to pathogen infection as determined by lesion size were recorded from samples harvested four months after inoculation, and least susceptible and highly susceptible genotypes were selected from the study material. Analysis of terpenoids from both least susceptible and highly susceptible pine genotypes by gas chromatography coupled with mass spectrometry indicates that some monoterpenes and sesquiterpenes are differentially induced depending on the susceptibility level. Transcriptomic microarray analysis was therefore conducted with RNA from stems of the least susceptible and highly susceptible Scots pine genotypes. Gene expression data from cDNA microarray were analysed by comparisons between the treatments, and the genotypes with different resistance level. The aim of the study is to highlight transcripts specific to differing levels of susceptibility.
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| Overall design |
We performed 24 hybridizations (NimbleGen) with samples derived from untreated trees (control) growing in drained peatland (3 replicates) and in mineral soil (3 replicates), artificially wounded trees growing in drained peatland (3 replicates) and in mineral soil (3 replicates), artificially infected trees growing in drained peatland (3 replicates) and in mineral soil (3 replicates). Replicate samples originate from the same individual tree. Samples were harvested 4 months after the inoculations. All samples were labeled with Cy3.
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| Contributor(s) |
Keriö S, Raffaello T, Kovalchuk A, Jaber E, Lorenz W, Holopainen J, Dean J, Asiegbu FO |
| Citation missing |
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| Submission date |
Feb 20, 2015 |
| Last update date |
Feb 02, 2019 |
| Contact name |
Tommaso Raffaello |
| E-mail(s) |
[email protected]
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| Organization name |
University of Helsinki
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| Department |
Forest Sciences
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| Street address |
Latokartanonkaari 7 PO Box 27
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| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
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| Platforms (1) |
| GPL19078 |
NimbleGen Pine 36K array v1.0 [110224_Pinus_FA_exp] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA276006 |
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