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Series GSE6629

Query DataSets for GSE6629

Status

Public on Sep 01, 2007

Title

Domain-Wide Regulation of Gene Expression in the Human Genome

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

Transcription factor complexes bind to regulatory sequences of genes, providing a system of individual expression regulation. Targets of distinct transcription factors usually map throughout the genome, without clustering. Nevertheless, highly and weakly expressed genes do cluster in separate chromosomal domains with an average size of 80 to 90 genes. We therefore asked whether, besides transcription factors, an additional level of gene expression regulation exists that acts on chromosomal domains. Here we show that identical green fluorescent protein (GFP) reporter constructs integrated at 90 different chromosomal positions determined by sequencing, obtain expression levels that correspond to the activity of the domains of integration. These domains are about 80 genes long and can exert an effect of up to 8-fold on the expression of integrated genes. 3D-FISH shows that active domains of integration have a more open chromatin structure than integration domains with weak activity. These results reveal a novel domain-wide regulatory mechanism that, together with transcription factors, exerts a dual control over gene transcription.
Keywords: HEK293 cells

Overall design

The experiment was performed in duplo, whereby HEK293 cells were grown under standard conditions and RNA was isolated, labelled and hybridized separately. Data from the duplicate arraying experiment were then averaged and further analyzed.

Contributor(s)

Gierman HJ, Indemans MH, Koster J, Goetze S, Seppen J, Geerts D, van Driel R, Versteeg R

Citation(s)

17693573

Submission date

Jan 03, 2007

Last update date

Mar 25, 2019

Contact name

Richard Volckmann

E-mail(s)

[email protected]