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| Status |
Public on Apr 29, 2015 |
| Title |
s54 -dependent response to nitrogen limitation and virulence in Burkholderia cenocepacia H111 |
| Organism |
Burkholderia cenocepacia H111 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Members of the genus Burkholderia are versatile bacteria capable of colonizing highly diverse environmental niches. In this study, we investigated the global response of the opportunistic pathogen Burkholderia cenocepacia H111 to nitrogen limitation at the transcript and protein expression level. In addition to a classical response to nitrogen starvation, including the activation of glutamine synthetase, PII proteins and the two component regulatory system ntrBC, B. cenocepacia H111 also up-regulated polyhydroxybutyrate (PHB) accumulation and exopolysaccharide (EPS) production in response to nitrogen shortage. A search for consensus sequences in promoter regions of nitrogen responsive genes identified a s54 consensus sequence. The mapping of the s54 regulon as well as the characterization of a s54 mutant suggests an important role of s54 not only in control of nitrogen metabolism, but also in virulence of this organism.
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| Overall design |
Nitrogen limitation and s54 regulon in B. cenocepacia
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| Contributor(s) |
Lardi M, Aguilar C, Pedrioli A, Suppiger A, Cárcamo-Oyarce G, Schmid N, Omasits U, Ahrens CH, Eberl L, Pessi G |
| Citation(s) |
25841012 |
| |
| Submission date |
Feb 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Gabriella Pessi |
| E-mail(s) |
[email protected]
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| Organization name |
University of Zürich
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| Street address |
Winterthurerstrasse 190
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| City |
Zürich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL16128 |
Illumina HiSeq 2000 (Burkholderia cenocepacia H111) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA276555 |
| SRA |
SRP055546 |
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