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| Status |
Public on Aug 28, 2015 |
| Title |
Transcriptome analysis of poplar during leaf spot infection with Sphaerulina spp. |
| Organisms |
Populus tremuloides; Populus deltoides; Populus balsamifera |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Diseases of poplar caused by the fungal pathogen Sphaerulina musiva and related species are of growing concern, particular with the increasing interest in intensive popluliculture to meet increasing energy demands. S. musiva is able to cause infection on leaves, resulting in defoliation and canker formation on stems. To gain a greater understanding of the different responses of poplar species to infection with their natural Sphaerulina species, RNA-seq was conducted on leaves of Populus deltoides, P. balsamifera and P. tremuloides infected with S. musiva, S. populicola and a new undescribed species Ston1, respectively. Progression of disease symptoms, pathogen growth and host response were detected. Through the time course of infection, different and species-specific metabolic pathways were activated. In all three species, genes associated with growth and development were down-regulated, while genes involved the phenylpropanoid, terpenoid and flavonoid biosynthesis were up-regulated. Poplar defensive genes were expressed early in P. balsamifera and P. tremuloides, but delayed in P. deltoides, which correlated with the rate of disease symptoms development. This data gives an insight into the large differences in timing and expression of genes between poplar species being attacked with their native associated Sphaerulina pathogen.
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| Overall design |
RNA-seq was conducted on leaves of Populus deltoides, P. balsamifera and P. tremuloides infected with S. musiva, S. populicola and a new undescribed species Ston1, respectively.
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| Contributor(s) |
Foster AJ, Pelletier G, Tanguay P, Séguin A |
| Citation(s) |
26378446 |
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| Submission date |
Apr 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Adam Foster |
| Organization name |
Natural Resources Canada
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| Department |
CFS
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| Lab |
Armand Seguin
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| Street address |
8678 rue de la Comtoise
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| City |
Quebec City |
| State/province |
Quebec |
| ZIP/Postal code |
G2C0L8 |
| Country |
Canada |
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| Platforms (3) |
| GPL20011 |
Illumina HiSeq 2000 (Populus balsamifera) |
| GPL20012 |
Illumina HiSeq 2000 (Populus deltoides) |
| GPL21118 |
Illumina HiSeq 2000 (Populus tremuloides) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA280709 |
| SRA |
SRP057004 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67697_EdgeR_poplar_RNAseq_table.xlsx |
19.2 Mb |
(ftp)(http) |
XLSX |
| GSE67697_P.balsamifera_de_novo_RNAseq_Counts_Table.xlsx |
300.1 Kb |
(ftp)(http) |
XLSX |
| GSE67697_P.balsamifera_de_novo_contigs.fasta.gz |
1.1 Mb |
(ftp)(http) |
FASTA |
| GSE67697_P.balsamifera_de_novo_edgeR_stats_table.xlsx |
758.0 Kb |
(ftp)(http) |
XLSX |
| GSE67697_P.deltoides_de_novo_RNAseq_Counts_Table.xlsx |
224.1 Kb |
(ftp)(http) |
XLSX |
| GSE67697_P.deltoides_de_novo_contigs.fasta.gz |
653.7 Kb |
(ftp)(http) |
FASTA |
| GSE67697_P.deltoides_de_novo_edgeR_stats_table.xlsx |
1.0 Mb |
(ftp)(http) |
XLSX |
| GSE67697_P.tremuloides_de_novo_RNAseq_Counts_Table.xlsx |
168.8 Kb |
(ftp)(http) |
XLSX |
| GSE67697_P.tremuloides_de_novo_contigs.fasta.gz |
551.0 Kb |
(ftp)(http) |
FASTA |
| GSE67697_P.tremuloides_de_novo_edgeR_stats_table.xlsx |
486.4 Kb |
(ftp)(http) |
XLSX |
| GSE67697_Poplar_RNAseq_Counts_Table.xlsx |
7.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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