|
| Status |
Public on Sep 30, 2015 |
| Title |
Gene expression data of regenerated human epidermis overexpressing control LACZ or SOX2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
SOX2 is not expressed in normal human epidermis but its expression is elevated in pre-neoplastic epidermis suggesting that it may have a role in the tumorigenesis process. The goal of this study is to determine the impacts of SOX2 overexpression in regenerated human epidermis.
Global gene expression profiling using U133 plus 2.0 arrays were used to deteremine the genes perturbed by overexpression of SOX2.
|
| |
|
| Overall design |
For gene expression profiling, cultured primary human keratinocytes were transduced to overexpress LACZ or SOX2. These genetically modified keratinocytes were used to regenerate human epidermis.RNA was harvested from regenerated human epidermis 6 days post-seeding of the cells. Microarray analysis using Affymetrix HG-U133 2.0 plus arrays was performed on duplicate samples.
|
| |
|
| Contributor(s) |
Chen Y, Sen GL |
| Citation(s) |
26344931 |
| |
| Submission date |
Apr 20, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
George Sen |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSD
|
| Department |
Dermatology/CMM
|
| Street address |
9500 GIlman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (4)
|
| GSM1662287 |
LACZ expressing Control, Biological Replicate 1 |
| GSM1662288 |
LACZ expressing control, Biological Replicate 2 |
| GSM1662289 |
SOX2 expressing Biological Replicate 1 |
|
| Relations |
| BioProject |
PRJNA281661 |
Less...
More...