|
| Status |
Public on Jun 05, 2015 |
| Title |
Structural evidence for Scc4-dependent localization of cohesin loading |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: The goal of this study is to look at differences in genome wide localisation of Scc1 cohesin between wild type and scc4-m35 in metaphase arrested cells.
Method: Wild-type (AM1145), wild type constructed in the same way as the mutant strain (carrying a HIS3 marker) (AM15540) and scc4-m35 mutant (AM15537) cells carrying SCC1-6HA were synchronised with a-factor and arrested in mitosis by treatment with nocodazole/benomyl for 2 hr. Samples were harvested, anti-HA ChIP was performed and input and IP samples were sequenced for the three strains.
Results: All 16 centromeres were specifically depleted of Scc1 in the scc4-m35 background. Scc1 depletion extended roughly 10 kilobases to either side of the core centromere but not to chromosome arms
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| |
|
| Overall design |
ChIP-seq for Scc1-6HA in metaphase-arrested cells in Wild type and in scc4-m35 mutant (samples VM5-VM10)
Vasso Makrantoni: experiments and Alastair Kerr: ChIP Seq Data analysis
https://github.com/AlastairKerr/Hinshaw2015
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| |
|
| Contributor(s) |
Makrantoni V, Kerr A |
| Citation(s) |
26038942 |
| |
| Submission date |
May 05, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Alastair Robert Kerr |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Centre for Cell Biology
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
2.21 Michael Swann Building, Kings Buildings, Mayfield Road
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (6)
|
| GSM1675773 |
AM1145_Scc1-6HA_2hrNocodazole_Input |
| GSM1675774 |
AM15537_Scc1-6HA_2hrNocodazole_scc4-m35::HIS3_Input |
| GSM1675775 |
AM15540_Scc1-6HA_2hrNocodazole_SCC4::HIS3_Input |
| GSM1675776 |
AM1145_Scc1-6HA_2hrNocodazole_IP |
| GSM1675777 |
AM15537_Scc1-6HA_2hrNocodazole_scc4-m35::HIS3_IP |
| GSM1675778 |
AM15540_Scc1-6HA_2hrNocodazole_SCC4::HIS3_IP |
|
| Relations |
| BioProject |
PRJNA283124 |
| SRA |
SRP058010 |
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