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| Status |
Public on May 19, 2015 |
| Title |
Expression analysis of Listeria monocytogenes LO28 delta-lhrC1-5 mutant and LO28 wt |
| Platform organism |
Listeria monocytogenes |
| Sample organism |
Listeria monocytogenes LO28 |
| Experiment type |
Expression profiling by array
|
| Summary |
Investigation of whole genome gene expression level changes in Listeria monocytogenes LO28 delta-lhrC1-5 mutant, compared to the wild type strain. The lhrC1-5 genes encode the regulatory sRNAs LhrC1-5. The microarray studied the gene expression of unstressed cells and cells exposed to cefuroxime for 30 min.
The lhrC1-5 mutant employed in this study is further described in Sievers et al. (2014) A multicopy sRNA of Listeria monocytogenes regulates expression of the virulence adhesin LapB. Nucleic Acids Res. 42:9383-98.
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| Overall design |
A twelve chip study using RNA extracted from 6 different cultures of L. monocytogenes LO28 delta-lhrC1-5 (three unstressed and three stressed cultures) and 6 different cultures of L. monocytognes LO28 wild-type (three unstressed cultures and three stressed cultures).
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| Contributor(s) |
Kallipolitis BH, Sievers S |
| Citation(s) |
26176322 |
| |
| Submission date |
May 18, 2015 |
| Last update date |
Aug 19, 2015 |
| Contact name |
Birgitte Haar Kallipolitis |
| Organization name |
University of Southern Denmark
|
| Department |
Biochemistry and Molecular Biology
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| Street address |
Campusvej 55
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| City |
Odense |
| ZIP/Postal code |
DK-5230 |
| Country |
Denmark |
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| Platforms (1) |
| GPL18538 |
NimbleGen Listeria monocytogenes 3K |
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| Samples (12)
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| GSM1689814 |
L_monocytogenes_LO28_wild-type_unstressed_rep1 |
| GSM1689815 |
L_monocytogenes_LO28_wild-type_unstressed_rep2 |
| GSM1689816 |
L_monocytogenes_LO28_wild-type_unstressed_rep3 |
| GSM1689817 |
L_monocytogenes_LO28_wild-type_stressed_rep1 |
| GSM1689818 |
L_monocytogenes_LO28_wild-type_stressed_rep2 |
| GSM1689819 |
L_monocytogenes_LO28_wild-type_stressed_rep3 |
| GSM1689820 |
L_monocytogenes_LO28_delta_lhrC1-5_unstressed_rep1 |
| GSM1689821 |
L_monocytogenes_LO28_delta_lhrC1-5_unstressed_rep2 |
| GSM1689822 |
L_monocytogenes_LO28_delta_lhrC1-5_unstressed_rep3 |
| GSM1689823 |
L_monocytogenes_LO28_delta_lhrC1-5_stressed_rep1 |
| GSM1689824 |
L_monocytogenes_LO28_delta_lhrC1-5_stressed_rep2 |
| GSM1689825 |
L_monocytogenes_LO28_delta_lhrC1-5_stressed_rep3 |
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| Relations |
| BioProject |
PRJNA284270 |
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