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| Status |
Public on Dec 01, 2016 |
| Title |
Naïve iPSCs Generated from β-thalassemia Fibroblasts Allow Efficient Gene Correction with CRISPR/Cas9 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We used a modified 5i/L/FA system to generate transgene-free naïve iPSCs directly from the fibroblasts of a patient suffering from β-thalassemia and further demonstrated efficient gene correction with a CRISPR/Cas9 system, which provides an improved strategy for personalized treatment of β-thalassemia.
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| Overall design |
A modified 5i/L/FA system was used to generate transgene-free naïve iPSCs directly from the fibroblasts of a patient suffering from β-thalassemia.
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| Contributor(s) |
Yang Y, Zhang X, Yi L, Hou Z, Chen J, Kou X, Zhao Y, Wang H, Sun X, Jiang C, Wang Y, Gao S |
| Citation(s) |
26676643 |
| |
| Submission date |
May 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaobai Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Tongji University
|
| Street address |
1239 Siping Road
|
| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA285174 |
| SRA |
SRP058802 |
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