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| Status |
Public on Jun 22, 2015 |
| Title |
miRNA profile in mice lung after influenza A infection |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The regulation of host defense against influenza A viruses (IAVs) infection has attracted much attention, especially for type I interferon (IFN)-mediated innate response. Here we revealed that miR-93 expression was significantly downregulated in Alveolar epithelial type II cells (AT2) upon IAVs infection through RIG-I/JNK pathway. Inhibition of miR-93 was found to suppress host antiviral innate response by facilitating type I IFN effector signaling, and JAK1 was identified to be directly targeted by miR-93. Importantly, in vivo administration of miR-93 antagomiR significantly inhibited miR-93 expression and markedly suppressed IAVs infection, which in turn prevented the death of IAVs infected mice. Hence, the inducible downregulation of miR-93 suppress IAVs infection by upregulation IFN-JAK-STAT effector pathway, and in vivo inhibition of miR-93 bears considerable therapeutic potential for suppressing IAVs infection.
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| Overall design |
The miRNA profiling in mice lung was measured at 24 and 36 hours after gave each mouse 50µl of influenza A (50 µl of 10-6 TCID50/µl) via retropharyngeal instillation. Three mice were performed at each time (24 or 36 hours) and RNA from different donors was mixed before determination.
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| Contributor(s) |
Wang Q, Guo M |
| Citation missing |
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| Submission date |
Jun 22, 2015 |
| Last update date |
Sep 27, 2016 |
| Contact name |
Meng Guo |
| E-mail(s) |
[email protected]
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| Phone |
+86-13916404693
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| Organization name |
ShangHai Changzheng Hospital
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| Department |
Organ transplantation center
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| Street address |
No. 415, Fengyang Road, Huangpu District
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| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL17912 |
Agilent-046065 Mouse_miRNA_V19.0_Microarray (miRNA ID version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA287682 |
