|
| Status |
Public on Jul 21, 2016 |
| Title |
localization of Scc2 between wild type and scc4-m35 cells in S-phase |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Wild-type (AM15307) and scc4-m35 (AM15311) cells carrying SCC2-3FLAG were fixed for 2 hr, 15 min following an a-factor arrest. Samples were harvested, anti-HA ChIP was performed and both input and IP samples were sequenced for both strains.
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| |
|
| Overall design |
ChIP-seq for S. cerevisiae Scc2-3FLAG in S-phase cells in Wild type and in scc4-m35 mutant
Vasso Makrantoni: experiments and Alastair Kerr: ChIP Seq Data analysis
|
| Web link |
https://github.com/AlastairKerr/Termanis2015
|
| |
|
| Contributor(s) |
Makrantoni V, Kerr A |
| Citation(s) |
27179028 |
| |
| Submission date |
Jun 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Alastair Robert Kerr |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Centre for Cell Biology
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
2.21 Michael Swann Building, Kings Buildings, Mayfield Road
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (4)
|
| GSM1675769 |
AM15307_Scc2-3xFLAG_SCC4::HIS3_S-Phase_Input |
| GSM1675770 |
AM15311_Scc2-3xFLAG_scc4-m35::HIS3_S-Phase_Input |
| GSM1675771 |
AM15307_Scc2-3xFLAG_SCC4::HIS3_S-Phase_IP |
| GSM1675772 |
AM15311_Scc2-3xFLAG_scc4-m35::HIS3_S-Phase_IP |
|
| Relations |
| BioProject |
PRJNA288305 |
| SRA |
SRP061275 |
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