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| Status |
Public on Aug 07, 2015 |
| Title |
Remodeling of Brown and White Adipose Tissue by NT-PGC-1α-Mediated Gene Regulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The β-adrenergic receptor signaling pathway is a major component of adaptive thermogenesis in brown and white adipose tissue during cold acclimation. The β-AR activation highly induces transcriptional coactivator PGC-1α and its splice variant N-terminal (NT)-PGC-1α, promoting the transcription program of mitochondrial biogenesis and thermogenesis. In the present study, we evaluated the role of NT-PGC-1α in brown adipocyte energy metabolism by genome-wide profiling of NT-PGC-1α-responsive genes. Canonical pathway analysis revealed that a number of genes upregulated by NT-PGC-1α are highly enriched in mitochondrial pathways including fatty acid transport and β-oxidation, TCA cycle and electron transport system, thus reinforcing the crucial role of NT-PGC-1α in the enhancement of mitochondrial function. Moreover, gene expression profiling of NT-PGC-1α revealed activation of distinct metabolic pathways such as glucose, lipid and nucleotide metabolism and of signaling pathways such as RAR and PPAR-γ/RXRα activation in brown adipocytes. Together, our data strengthen our previous findings that NT-PGC-1α is a key regulator of mitochondrial oxidative metabolism and thermogenesis in brown adipocytes and further suggest that NT-PGC-1α influences a broader spectrum of thermogenic processes to meet cellular needs for adaptive thermogenesis.
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| Overall design |
Two samples from two groups: NT-PGC-1α overexpression and empty vector. There are technical replicates (A and B) for each group. Two RNA samples were pooled for each group.
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| Contributor(s) |
Kim J, Fernand VE, Henagan T, Shin J, Huypens P, Newman S, Gettys TW, Chnag JS |
| Citation(s) |
27454177 |
| |
| Submission date |
Aug 06, 2015 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Ji Suk Chang |
| Organization name |
Pennington Biomedical Research Center
|
| Department |
Gene Regulation and Metabolism
|
| Street address |
6400 Perkins Rd.
|
| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
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| Platforms (1) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA292062 |
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