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| Status |
Public on May 28, 2016 |
| Title |
Gene expression signatures in cell cycle sorted cells [HeLa_mRNA_array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
For the further examination of cell cycle dependent gene expression profile, DNA content based fluorescence activated cell sorting (cell cycle sort) was performed on human transformed cancer cell lines and primary, untransformed fibroblasts. Phase-dependent gene expression profiling was performed on G1, S and G2 phases. Results were validated by quantitative real-time PCR.
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| Overall design |
Phase-dependent gene expression profiling was performed on sorted human cancer (cervical - HeLa and adrenocortical - NCI-H295R) cells and primary untransformed fibroblasts (HDFa). G1, S and G2 phases were successfully sorted and analyzed.
HeLa_mRNA dataset
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| Contributor(s) |
Grolmusz VK, Patocs A |
| Citation(s) |
27234232 |
| |
| Submission date |
Sep 18, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Vince Kornél Grolmusz |
| Organization name |
Semmelweis University
|
| Street address |
Szentkirályi 46.
|
| City |
Budapest |
| ZIP/Postal code |
1088 |
| Country |
Hungary |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE73259 |
Fluorescence activated cell sorting followed by small RNA sequencing reveals stable microRNA expression during cell cycle progression |
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| Relations |
| BioProject |
PRJNA296356 |
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