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Series GSE7350 Query DataSets for GSE7350
Status Public on Nov 20, 2007
Title CodY of Streptococcus pneumoniae: link between nutritional gene regulation and virulence
Platform organism Streptococcus pneumoniae TIGR4
Sample organisms Streptococcus pneumoniae; Streptococcus pneumoniae TIGR4
Experiment type Expression profiling by array
Summary CodY is a nutritional regulator mainly involved in amino acid metabolism. It has been extensively studied in Bacillis subtilis and Lactococcus lactis. We investigated the role of CodY in gene regulation and virulence of the human pathogen Streptococcus pneumoniae. We constructed a codY-mutant and examined the effect on gene and protein expression by microarray and 2D DIGE analysis. The pneumococcal CodY-regulon was found to consist predominantly of genes involved in amino acid metabolism, but also several other cellular processes, such as carbon metabolism and iron uptake. By means of electrophoretic mobility shift assays and DNA footprinting, we showed that most targets identified are under direct control of CodY. By mutating DNA predicted to represent the CodY-box based on the L. lactis consensus, we demonstrated that this sequence is indeed required for in vitro DNA-binding to target promoters. Similar to L. lactis, DNA-binding of CodY was enhanced in the presence of the branched chain amino acids isoleucine, leucine, and valine, and not by GTP. We observed in experimental mouse models that CodY is transcribed in the murine nasopharynx and lungs, and is specifically required for colonization. This finding was underscored by the diminished ability of the codY-mutant to adhere to nasopharyngeal cells in vitro. In conclusion, pneumococcal CodY predominantly regulates genes involved in amino acid metabolism and contributes to the early stages of infection, i.e. colonization of the nasopharynx.
Keywords: codY
 
Overall design CodY of Streptococcus pneumoniae: link between nutritional gene regulation and virulence
 
Contributor(s) Hendriksen W
Citation(s) 18024519
Submission date Mar 23, 2007
Last update date Mar 17, 2012
Contact name Anne de Jong
E-mail(s) [email protected]
Phone +31 50 363 2047
Organization name university of Groningen
Department Molecular Genetics
Street address Nijenborgh 7
City Groningen
ZIP/Postal code 9747 AG
Country Netherlands
 
Platforms (1)
GPL5023 Streptococcus pneumoniae TIGR4 v6 codY
Samples (7)
GSM176875 codY S.pneu TIGR4 164681
GSM176877 codY S.pneu TIGR4 164682
GSM177084 codY S.pneu TIGR4 164683
Relations
BioProject PRJNA100417

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Supplementary file Size Download File type/resource
GSE7350_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)

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