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Status |
Public on Nov 20, 2007 |
Title |
CodY of Streptococcus pneumoniae: link between nutritional gene regulation and virulence |
Platform organism |
Streptococcus pneumoniae TIGR4 |
Sample organisms |
Streptococcus pneumoniae; Streptococcus pneumoniae TIGR4 |
Experiment type |
Expression profiling by array
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Summary |
CodY is a nutritional regulator mainly involved in amino acid metabolism. It has been extensively studied in Bacillis subtilis and Lactococcus lactis. We investigated the role of CodY in gene regulation and virulence of the human pathogen Streptococcus pneumoniae. We constructed a codY-mutant and examined the effect on gene and protein expression by microarray and 2D DIGE analysis. The pneumococcal CodY-regulon was found to consist predominantly of genes involved in amino acid metabolism, but also several other cellular processes, such as carbon metabolism and iron uptake. By means of electrophoretic mobility shift assays and DNA footprinting, we showed that most targets identified are under direct control of CodY. By mutating DNA predicted to represent the CodY-box based on the L. lactis consensus, we demonstrated that this sequence is indeed required for in vitro DNA-binding to target promoters. Similar to L. lactis, DNA-binding of CodY was enhanced in the presence of the branched chain amino acids isoleucine, leucine, and valine, and not by GTP. We observed in experimental mouse models that CodY is transcribed in the murine nasopharynx and lungs, and is specifically required for colonization. This finding was underscored by the diminished ability of the codY-mutant to adhere to nasopharyngeal cells in vitro. In conclusion, pneumococcal CodY predominantly regulates genes involved in amino acid metabolism and contributes to the early stages of infection, i.e. colonization of the nasopharynx. Keywords: codY
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Overall design |
CodY of Streptococcus pneumoniae: link between nutritional gene regulation and virulence
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Contributor(s) |
Hendriksen W |
Citation(s) |
18024519 |
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Submission date |
Mar 23, 2007 |
Last update date |
Mar 17, 2012 |
Contact name |
Anne de Jong |
E-mail(s) |
[email protected]
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Phone |
+31 50 363 2047
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Organization name |
university of Groningen
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Department |
Molecular Genetics
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Street address |
Nijenborgh 7
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City |
Groningen |
ZIP/Postal code |
9747 AG |
Country |
Netherlands |
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Platforms (1) |
GPL5023 |
Streptococcus pneumoniae TIGR4 v6 codY |
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Samples (7)
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Relations |
BioProject |
PRJNA100417 |