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| Status |
Public on Oct 30, 2015 |
| Title |
GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae |
| Organism |
Streptococcus pneumoniae D39 |
| Experiment type |
Expression profiling by array
|
| Summary |
We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of Δ galK with the D39 wildtype strain grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| Citation(s) |
26544195 |
| |
| Submission date |
Oct 29, 2015 |
| Last update date |
Nov 02, 2016 |
| Contact name |
Sulman Shafeeq |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Department |
MTC
|
| Street address |
nobel vag 16
|
| City |
STOCKHOLM |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL11484 |
Streptococcus pneumoniae D39 Multiplatform 1909k array |
|
| Samples (4)
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| This SuperSeries is composed of the following SubSeries: |
| GSE74500 |
Impact of galR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose |
| GSE74501 |
Impact of galR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Galactose |
|
| Relations |
| BioProject |
PRJNA300565 |
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