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Series GSE7510 Query DataSets for GSE7510
Status Public on Apr 09, 2008
Title Acute graft-versus-host disease (gene expression profiling)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Allogeneic hematopoietic stem cell transplantation (HSCT) is the treatment of choice for high-risk hematological malignancies, yet a major complication associated with this therapy is acute graft-versus-host disease (GVHD). Despite a well-defined pathophysiological mechanism, there are no definitive markers for predicting acute GVHD development or progression to advanced stages. In the current study, we enrolled four acute GVHD and four acute GVHD-free recipients of allogeneic HSCT and collected peripheral blood just prior to onset of clinical acute GVHD for analysis on Affymetrix GeneChip Human Genome U133 Plus 2.0 microarrays. We noted significant differences in expression of 1,658 genes between control and acute GVHD patients, based on an analysis of covariance (ANCOVA) by type of transplant, a pooled error estimate, and a false discovery rate (FDR) of 10%. In conclusion, we offer the first report of a preliminary molecular signature of acute GVHD in allogeneic HSCT patients.
 
Overall design Samples 1-4 are control patients and samples 5-8 are acute GVHD patients. Peripheral blood was collected at engraftment and used for microarray analysis. The type of transplant (unrelated/related donor) was used as a covariate to assess if there were significant changes in gene expression between the control (1-4) and acute GVHD (5-8) groups, based on a 10% FDR with a pooled error estimate.
 
Contributor(s) Buzzeo M, Reddy V
Citation(s) 18714668
Submission date Apr 12, 2007
Last update date Mar 25, 2019
Contact name Matthew Buzzeo
E-mail(s) [email protected]
Organization name University of South Florida College of Medicine
Street address 12901 Bruce B. Downs Blvd
City Tampa
State/province FL
ZIP/Postal code 33612
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (8)
GSM182021 1 MUD Ctrl
GSM182022 2 MUD Ctrl
GSM182023 3 related Ctrl
Relations
BioProject PRJNA100457

Differentially expressed genes header descriptions
Probe Set ID
Gene Title
Gene Symbol
Fold Change (Log2)
P (ANCOVA)
GO Biological Process Description
1_MUD_Ctrl
2_MUD_Ctrl
3_Related_Ctrl
4_Related_Ctrl
5_MUD_GVHD
6_MUD_GVHD
7_Related_GVHD
8_Related_GVHD

Data table
Probe Set ID Gene Title Gene Symbol Fold Change (Log2) P (ANCOVA) GO Biological Process Description 1_MUD_Ctrl 2_MUD_Ctrl 3_Related_Ctrl 4_Related_Ctrl 5_MUD_GVHD 6_MUD_GVHD 7_Related_GVHD 8_Related_GVHD
1561331_at chromosome 1 open reading frame 99 C1orf99 13.10 0.0022 --- 2.2 2.4 0.6 2.1 2.7 56.9 43 29.7
218800_at steroid 5 alpha-reductase 2-like SRD5A2L 10.86 0.0001 --- 2.2 8.6 36.1 4.2 53.4 92.6 84.1 95.8
1559653_at Growth hormone regulated TBC protein 1 GRTP1 10.77 0.0000 --- 1.9 3.5 11.7 4.8 18.7 66.6 46.1 87.4
233328_x_at chromosome 20 open reading frame 59 C20orf59 10.58 0.0000 transport 10.1 8 16.7 7 109.6 91.2 123.2 96
230067_at Hypothetical protein FLJ30707 FLJ30707 10.01 0.0000 --- 4.8 6.4 8.5 23.2 100.7 110.8 71.4 76.4
243119_at Nuclear fragile X mental retardation protein interacting protein 2 NUFIP2 9.51 0.0000 --- 2.9 20.8 4.1 12.6 46.7 111.8 66.8 73.1
215591_at SATB family member 2 SATB2 9.48 0.0001 regulation of transcription, DNA-dependent /// transcription /// regulation of transcription 26.4 4.3 8.8 1.9 49.9 80.8 53.6 70.8
220623_s_at testis specific, 10 TSGA10 9.19 0.0000 spermatogenesis 10 10.1 7.2 4.3 41 78.5 49.3 140.5
238919_at Protocadherin 9 PCDH9 9.14 0.0000 cell adhesion /// homophilic cell adhesion 15.1 3.3 5.4 2.6 33.7 49.2 55.3 53.2
226360_at zinc and ring finger 3 ZNRF3 8.80 0.0000 ubiquitin cycle 11.6 10 9.3 2.1 77.2 93.1 31.9 59.2
1570352_at ataxia telangiectasia mutated (includes complementation groups A, C and D) ATM 8.76 0.0003 DNA repair /// cell cycle /// meiotic recombination /// signal transduction /// negative regulation of progression through cell cycle /// regulation of progression through cell cycle /// DNA repair /// response to DNA damage stimulus 2.6 2.8 3.6 33.9 26.1 64.5 43.1 72.1
1569476_at DKFZP434L187 protein DKFZP434L187 8.68 0.0000 --- 4.5 8.8 11.9 6.2 58.1 84.6 45.2 74.6
207461_at --- --- 8.51 0.0000 chromatin assembly or disassembly /// transcription /// regulation of transcription, DNA-dependent /// chromatin modification /// regulation of transcription from RNA polymerase II promoter /// chromosome organization and biogenesis (sensu Eukaryota) 2.8 2 4.8 2.8 25 27.2 38.7 15
211187_at --- --- 8.28 0.0001 --- 3.2 27.8 2.9 2.8 127.4 39.3 23.3 29.1
1564192_at Supervillin SVIL 8.03 0.0009 cytoskeleton organization and biogenesis /// striated muscle development /// barbed-end actin filament capping /// transport 21.5 5.5 2.9 1.4 18.1 57.2 53 36.3
220184_at Nanog homeobox NANOG 7.62 0.0000 regulation of transcription, DNA-dependent /// regulation of transcription /// transcription 13.3 2.7 10.8 4.1 63.4 67.6 21.9 57.1
221458_at 5-hydroxytryptamine (serotonin) receptor 1F HTR1F 7.54 0.0000 signal transduction /// G-protein signaling, coupled to cyclic nucleotide second messenger /// synaptic transmission /// G-protein coupled receptor protein signaling pathway 9.6 21.2 13 5.4 103.7 37.7 117.4 100.8
1570293_at transducin (beta)-like 1X-linked TBL1X 7.27 0.0012 transcription /// regulation of transcription, DNA-dependent /// ubiquitin cycle /// signal transduction /// visual perception /// sensory perception of sound /// sensory perception of sound 16.3 8.2 3.3 3.9 13.5 84.3 85.6 49.4
227691_at A kinase (PRKA) anchor protein 8-like AKAP8L 7.21 0.0000 --- 12.5 7 12.3 24.3 58 126.3 75.5 128
215254_at Down syndrome critical region gene 1 DSCR1 7.16 0.0000 signal transduction /// central nervous system development /// circulation /// calcium-mediated signaling 17.5 3.8 11.2 3.4 88 65.1 44.1 26.3

Total number of rows: 1658

Table truncated, full table size 275 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE7510_(supplementary)_GVHD_pooled_error_FDR10percent_annotated.xls 473.5 Kb (ftp)(http) XLS
GSE7510_RAW.tar 36.0 Mb (http)(custom) TAR (of CEL)

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