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| Status |
Public on Dec 01, 2015 |
| Title |
Transposon sequencing during experimental osteomyelitis reveals hypoxic responses as key components of the staphylococcal-host interaction |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by array
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| Summary |
The Staphylococcus aureus two-component regulatory system, SrrAB, coordinates hypoxic responses during in vitro growth conditions.
S. aureus Affymetrix GeneChips were used to compare S. aureus expression properties of wild type and isogenic ssrA mutant cells during aerobic growth conditions.
S. aureus Affymetrix GeneChips were also used to compare the S. aureus expression properties of wild type and isogenic ssrA mutant cells during hypoxic growth conditions.
Few differences were observed between the expression properties of S. aureus wildtype and ssrA mutant cells during aerobic growth.
Significant differences were observed between the expression properties of S. aureus wild type and ssrA mutant cells during hypoxic growth.
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| Overall design |
S. aureus strain USA300 (wild type) or an isogenic srrAB mutant (DssrA) were grown in laboratory medium to exponential under aerobic or hypoxic growth conditions, total bacterial RNA was isolated, labeled and applied to Affymetrix GeneChips. We sought to determine whether the transcriptional profiles of S. aureus wild type and ssrAB mutant cells differed during aerobic and hypoxic growth conditions.
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| Contributor(s) |
Dunman PM, Cassat J |
| Citation(s) |
26684646 |
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| Submission date |
Nov 30, 2015 |
| Last update date |
Mar 01, 2016 |
| Contact name |
Paul Dunman |
| E-mail(s) |
[email protected]
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| Phone |
585-276-5700
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| Organization name |
University of Rochester
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| Department |
Microbiology and Immunology
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| Street address |
601 Elmwood Avenue
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL1339 |
[S_aureus] Affymetrix S. aureus Genome Array |
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| Samples (8)
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| GSM1958422 |
strain USA300-Lac, planktonic aerobic growth, rep 1 |
| GSM1958423 |
strain USA300-Lac, planktonic aerobic growth, rep 2 |
| GSM1958424 |
strain USA300-Lac, planktonic hypoxic growth, rep 1 |
| GSM1958425 |
strain USA300-Lac, planktonic hypoxic growth, rep 2 |
| GSM1958426 |
strain ssrAB, planktonic aerobic growth, rep 1 |
| GSM1958427 |
strain ssrAB, planktonic aerobic growth, rep 2 |
| GSM1958428 |
strain ssrAB, planktonic hypoxic growth, rep 1 |
| GSM1958429 |
strain ssrAB, planktonic hypoxic growth, rep 2 |
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| Relations |
| BioProject |
PRJNA304450 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75516_RAW.tar |
8.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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