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| Status |
Public on Dec 01, 2018 |
| Title |
RNA-sequencing of wild-type (HHY168 background), REB1-FRB, and BDP1-FRB Saccharomyces cerevisiae strains |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
FRB-tagged Reb1p and Bdp1p were depleted from the nucleus using the anchor away technique (Haruki et al., Mol Cell, 2008, 31:925-932), and RNA-sequencing was used to assay the impact of these factors on alternative poly(A) site selection.
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| |
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| Overall design |
RNA-sequencing of biological replicates of WT, REB1-FRB and BDP1-FRB treated for 60 minutes with rapamycin.
Biological replicates of WT, REB1-FRB and BDP1-FRB treated for 60 minutes with rapamycin.
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| |
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| Contributor(s) |
Roy K, Chanfreau G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Roy |
| E-mail(s) |
[email protected]
|
| Organization name |
UCLA
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Guillaume Chanfreau
|
| Street address |
607 Charles E. Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA304630 |
| SRA |
SRP066858 |
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