|
| Status |
Public on Aug 08, 2016 |
| Title |
Transcriptomic responses of copepodid sea lice (Lepeophtheirus salmonis) exposed to cypermethrin (Betamax®) |
| Organism |
Lepeophtheirus salmonis |
| Experiment type |
Expression profiling by array
|
| Summary |
This study investigates transcriptomic responses of Atlantic salmon lice, Lepeophtheirus salmonis exposed to cypermethrin, a commonly used antiparasitic agent used in aquaculture. Copepodid L. salmonis were exposed to cypermethrin (Betamax®) at a concentration of 1.0ppb
|
| |
|
| Overall design |
An in vitro bioassay experiment was conducted using cypermethrin exposures on copepodid (larvae) sea lice (F1 generation) collected from BMA-2a New Brunswick, Canada in 2014. The bioassay exposed copepodids to 1.0 μg/L cypermethrin or a sea water control for 24 hours in glass beakers (VWR) at 12 ± 1oC. Each condition had a total volume of 500mL with six replicates and approximately 500 lice per beaker. After 24 hours, pools of ~500 copepodids were flash frozen for RNA extractions. Post 24-hours, lice were assessed for survival similarly to the technique used for staging and enumeratio first
|
| |
|
| Contributor(s) |
Poley JD, Braden LM, Whyte SK, Messmer A, Koop BF, Fast MD |
| Citation(s) |
27612154 |
| |
| Submission date |
Jan 05, 2016 |
| Last update date |
Nov 07, 2016 |
| Contact name |
Ben F Koop |
| E-mail(s) |
[email protected]
|
| Phone |
(250) 472-4067
|
| Organization name |
The University of Victoria
|
| Department |
Biology
|
| Lab |
Centre for Biomedical Research
|
| Street address |
PO Box 3020 STN CSC
|
| City |
Victoria |
| State/province |
BC |
| ZIP/Postal code |
V8W 3N5 |
| Country |
Canada |
| |
|
| Platforms (1) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA307840 |
Less...
More...