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| Status |
Public on May 01, 2007 |
| Title |
Gene expression profile after doxycycline treatment in keratin5 knockout epidermis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Epidermolysis bullosa simplex (EBS) is a skin disorder caused by mutations in keratin (K) 5 or K14 genes. Little effect therapy is available.Following clinical reports, we applied the small molecule doxycycline to K5-/- mice. We demonstrate that doxycycline extended the survival of neonatal K5-/- mice from less than 1 to up to 8 hours. Microarray and TaqMan real-time PCR showed a downregulation of MMP-13 and IL-1beta, indicating an effect of doxycycline on transcription. Our data offer a novel small molecule based therapy approach for EBS.
Keywords: drug treatment, global gene expression comparative profile
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| Overall design |
doxycycline (50 µg/mlwith 5 % sucrose) was administered by oral route to pregnant K5+/- females (mated to K5+/- males) starting from E13.5. 5 % sucrose solution was administered to the control group.
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| Contributor(s) |
Lu H, Chen J, Planko L, Zigrino P, Klein-Hitpass L, Magin TM |
| Citation missing |
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| |
| Submission date |
Apr 30, 2007 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Hong Lu |
| Organization name |
University of Bonn
|
| Street address |
Nussallee 11
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| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA99741 |
