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| Status |
Public on May 16, 2016 |
| Title |
RNA-seq expression data from EB-HSPCs after HOXA7 overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
HOXA7 regulates FL-HSPC self-renewal in vitro and in vivo. We profiled EB-HSPCs after HOXA7 overexpression (EB-HOXA7), or with a control vector (EB-CTR), to assess the gene expression programs regulated by HOXA7.
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| Overall design |
CD34+CD38-CD43+CD90+ HSPCs were infected with lentiviral FUGW vector either empty (FUGW-GFP) or encoding HOXA7(FUGW-GFP-HOXA7) protein. Cells were expanded on op9 for 15 days and than sorted for GFP HSPC immunophenotype.
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| Contributor(s) |
Dou DR, Calvanese V, Sierra MI, Nguyen AT, Minasian A, Saarikoski P, Sasidharan R, Ramirez CM, Zack JA, Crooks GM, Galic Z, Mikkola HK |
| Citation(s) |
27183470 |
| |
| Submission date |
Jan 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hanna Katri Annikki Mikkola |
| E-mail(s) |
[email protected]
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| Organization name |
University of California, Los Angeles
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| Department |
Molecular, Cell and Developmental Biology
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| Lab |
Mikkola Lab
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| Street address |
621 Charles E Young Drive South, 2204, Mailbox 160606
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90092 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
| GSM2035730 |
EB cells infected with the empty FUGW-GFP |
| GSM2035731 |
EB cells infected with FUGW-GFP-HOXA7 |
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| This SubSeries is part of SuperSeries: |
| GSE76685 |
Medial HOXA gene expression is a landmark for the definitive haematopoietic fate and a prerequisite for human HSC function |
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| Relations |
| BioProject |
PRJNA308424 |
| SRA |
SRP068281 |
