|
| Status |
Public on Jan 12, 2016 |
| Title |
The Histone Methyltransferases MLL1 and DOT1L Cooperate with Meningioma-1 to Induce AML [Human ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: To characterize the genome-wide distribution of H3K79me2 in human leukemia cell lines treated with the Dot1l inhibitor EPZ004777 or control
Methods: We performed Chip-seq for the H3K79me2 on the leukemia cell lines Mutz3, Loucy and Molm14 after 6 days in culture in the presence of 3uM EPZ004777 or DMSO control
Results: H3K79me2 is completely erased from key target genes such as the HOXA cluster.
Conclusions: Exposure of Mutz3, Loucy and Molm14 to 3uM EPZ004777 erases H3K79 methylation globally as well as on key loci
|
| |
|
| Overall design |
ChIP-Seq for H3K79me2 on human leukemia cell lines exposed for 6 days to the Dot1l inhibitor EPZ004777
|
| |
|
| Contributor(s) |
Riedel S, Neff T, Bernt K |
| Citation(s) |
26927674 |
| |
| Submission date |
Jan 11, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Tobias Neff |
| E-mail(s) |
[email protected]
|
| Phone |
303-724-7445
|
| Organization name |
University of Colorado Denver AMC
|
| Department |
Pediatric Oncology
|
| Lab |
Neff
|
| Street address |
12800 E. 19th Ave.
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE76750 |
The Histone Methyltransferases MLL1 and DOT1L Cooperate with Meningioma-1 to Induce AML |
|
| Relations |
| BioProject |
PRJNA308491 |
| SRA |
SRP068316 |
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