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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 20, 2007 |
| Title |
miRNA34 expression in SW480 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
SW480 were stably transfected with an episomal plasmid expressing GFP and miRNA34 from a bidirectional doxycyclin regulatable promoter (Bornkamm et al Nucleic Acids Res. 2005 Sep 7;33(16)). Polyclonal cell lines were obtained by selection with Hygromycin at 350ug/ml for 10 days. The cell llnes identified as GFP only express GFP, whereas the cell lines identified as miRNA34a express both GFP and miRNA34 under doxycyclin control. For the present experiment, cells were treated with 1ug/ml Docycyclin for 72h. Cells were harvested and total RNA was isolated using Trizol (Invitrogen). After RNA cleanup (RNeasy, Qiagen) Affymetrix 133 Plus 2.0 micorarrays were hybridized using standard techniques.
Keywords: Cell line transfection
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| Overall design |
2 cell lines with transfected miRNA34a with doxycyclin treatment, 2 cell lines with miRNA34a but without doxycyclin treatment, 2 cell lines with transfected GFP with doxycyclin treatment, and 2 cell lines with GFP but without doxycylin treatment. One array for per sample.
The log-transformed probe-set values and the results of the statistical analysis for each probe-set, and the associated README file, are included as Supplementary files.
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| Contributor(s) |
Bommer GT, Feng Y, Kacsorowski A, Gerin I, Kuick R, Giordano T, MacDougald O, Cho KR, Fearon ER |
| Citation(s) |
17656095 |
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| Submission date |
May 01, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Rork Kuick |
| E-mail(s) |
[email protected]
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| Phone |
734-936-9241
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| Organization name |
University of Michigan
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| Street address |
University of Michigan, SPH II, 1415 Washington Heights, Room M2533
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2029 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| GSM186024 |
SW480_miRNA34a_no_Dox biological replicate 1 |
| GSM186025 |
SW480_miRNA34a_no_Dox biological replicate 2 |
| GSM186026 |
SW480_miRNA34a_with_Dox biological replicate 1 |
| GSM186027 |
SW480_miRNA34a_with_Dox biological replicate 2 |
| GSM186028 |
SW480_GFP_no_Dox biological replicate 1 |
| GSM186029 |
SW480_GFP_no_Dox biological replicate 2 |
| GSM186030 |
SW480_GFP_with_Dox biological replicate 1 |
| GSM186031 |
SW480_GFP_with_Dox biological replicate 2 |
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| Relations |
| BioProject |
PRJNA99475 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7678_MiRNA34cells_analysis_results.xls |
34.2 Mb |
(ftp)(http) |
XLS |
| GSE7678_RAW.tar |
45.2 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE7678_README.txt |
2.5 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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