|
| Status |
Public on Apr 08, 2010 |
| Title |
Genes regulated by YAP in normal breast cell line and breast cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Frequent loss of heterozygosity (LOH) at 11q22-23 in breast cancer strongly suggests that this region contains a tumor suppressor gene, yet to be identified. We and others have shown Yes-associated protein (YAP), which is located at 11q22.2, transactivates the p53 family member p73 upon DNA damage, suggesting a tumor suppressive function for YAP. Our analysis of breast tumor tissues by immunohistochemistry (IHC) showed loss of YAP protein expression in great portion of breast cancers.
We used microarray to look at the targte genes regulated by YAP in normal breast luminal cell and breast cancer cell lines.
Keywords: gene knock down
|
| |
|
| Overall design |
We generated stable cell lines by introducing control vector(pRS-IRES-GFP/pmig) or YAP shRNA(pRS-IRES-GFP-YAP/pmig-YAP) in a normal breast luminal cell line 1089 luminal and breast cancer cell lines MDA MB231. RAN was extracted and hybridized on Affymetrix microarrays.We looked for new target genes regulated by YAP in normal breast luminal cell and breast cancer cell lines.
|
| |
|
| Contributor(s) |
Yuan M, Holliday D, Chelala C, Harada T, Gangeswaran R, Ali S, Mein C, Lemoine NR, Jones L, Basu S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 03, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Subham Basu |
| E-mail(s) |
[email protected]
|
| URL |
http://science.cancerresearchuk.org/research/loc/london/clincentre_bartslondon/basus/
|
| Organization name |
Cancer Research UK
|
| Department |
Molecular Oncology Unit
|
| Lab |
Cell Survival Signalling Laboratory
|
| Street address |
Charterhouse Square
|
| City |
london |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (10)
|
| GSM187268 |
MDA MB231 control cell pmig/pRS-IRES-GFP rep1 |
| GSM187269 |
MDA MB231 control cell pmig/pRS-IRES-GFP rep2 |
| GSM187271 |
MDA MB231 YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep1 |
| GSM187272 |
MDA MB231 YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep2 |
| GSM187273 |
Normal luminal control cell pmig/pRS-IRES-GFP rep1 |
| GSM187274 |
Normal luminal control cell pmig/pRS-IRES-GFP rep2 |
| GSM187275 |
Normal luminal control cell pmig/pRS-IRES-GFP rep3 |
| GSM187276 |
Normal luminal YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep1 |
| GSM187277 |
Normal luminal YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep2 |
| GSM187278 |
Normal luminal YAP knock down cell pmig-YAP/pRS-IRES-GFP-YAP rep3 |
|
| Relations |
| BioProject |
PRJNA99467 |
Less...
More...