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| Status |
Public on Jan 03, 2008 |
| Title |
Halobacterium NRC-1 oxygen, light and htr2 perturbation |
| Organism |
Halobacterium salinarum NRC-1 |
| Experiment type |
Expression profiling by array
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| Summary |
A 50ml culture of Halobacterium NRC-1 knockout strain delta-ura3 with additional in-frame gene deletion of htr2 was grown to mid-log phase in a 125ml flask in rich media under varying oxygen and light conditions. Low and high light indicate growth in a painted flask versus growth in a standard clear flask under constant standard incubator illumination, respectively. Low and high oxygen indicate shaking in a stoppered flask at 100RPM agitation or shaking in a non-stoppered flask at 220RPM, respectively. Each of the four possible pairwise environemental combinations were assayed : High-oxygen/high-light, Low-oxygen/high-light, High-oxygen/low-light and Low-oxygen/low-light.
RNA extractions were performed using the Stratagene Absolutely RNA Miniprep Kit and RNA quality checked with the Agilent Bioanalyzer and with Oligo Microarrays were fabricated at the Institute for Systems Biology Microarray Facility. The arrays contain 4 spots per unique 70-mer oligonucleotides for each of 2400 non-redundant genes in Halobacterium NRC-1 Labeling, hybridization and washing have been previously described (Baliga et al. 2002) with 10 μg of RNA from the sample and reference. RNA from the final time point of a replicate experiment was used as the reference. Bias in dye incorporation was accounted for by reversing the labeling dyes (dye-flip). Raw data was processed and converted into log10 ratios with lambda (λ) values determined by a maximum likelihood method.Baliga, N. S., Pan, M., Goo, Y. A., Yi, E. C., Goodlett, D. R., Dimitrov, K., Shannon, P., Aebersold, R., Ng, W. V. & Hood, L. (2002) Proc Natl Acad Sci U S A 99:14913-84.
Keywords: environmental response, genetic perturbation
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| Overall design |
4 samples were analyzed in duplicate (8 total microarrays) as dye-flips.
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| Contributor(s) |
Pan M, Baliga NS |
| Citation(s) |
18160043 |
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| Submission date |
May 04, 2007 |
| Last update date |
Jan 15, 2017 |
| Contact name |
Marc Facciotti |
| E-mail(s) |
[email protected]
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| Organization name |
Institute for Systems Biology
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| Street address |
1441 North 34th Street
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
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| Platforms (1) |
| GPL3739 |
ISB Halobacterium Spotted Microarray |
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| Samples (8)
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| GSM187052 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6609 |
| GSM187053 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6610 |
| GSM187054 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6611 |
| GSM187055 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6612 |
| GSM187056 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6613 |
| GSM187057 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6614 |
| GSM187058 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6615 |
| GSM187059 |
Halobacterium oxygen and light perturbation in htr2 deletion strain. Array 6616 |
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| This SubSeries is part of SuperSeries: |
| GSE7740 |
Halobacterium NRC-1 oxygen, light and pertubations |
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| Relations |
| BioProject |
PRJNA105473 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7731_RAW.tar |
9.5 Mb |
(http)(custom) |
TAR (of CSV) |
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