 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 14, 2016 |
| Title |
Gene expression data from Mycobacterium tuberculosis-infected WT and miR-155-/- bone-marrow derived macrophages [validation] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
How the complex interaction between Mycobacterium tuberculosis (Mtb) and the host is regulated during infection is still not well understood. Using a systems biology approach, we demonstrate here that miR-155 is one of several microRNAs that regulate host gene expression over the first 48 hours of Mtb infection in macrophages. miR-155 regulates the cell survival of Mtb-infected macrophages through SHIP1/AKT signaling.
Using timecourse gene expression data, we constructed a miRNA regulatory network for the innate immune response to Mtb infection by WT macrophages. The network suggested a role for seven miRNAs in regulating the host response to Mtb, with miR-155 being one of them. We then validated a role for miR-155 by comparing the response between WT and miR-155-/- macrophages.
|
| |
|
| Overall design |
Three replicates of WT C57BL/6 BMMs were collected at 0, 4, 8, 24, and 48 hours post-infection with Mycobacterium tuberculosis, H37Rv strain (MOI 5). The regulatory network was then validated with WT and miR-155-/- BMM samples collected at 0 and 8 hours post-infection (three replicates each).
This dataset represents WT and miR-155-/- BMM samples collected at 0 and 8 hours post-infection (three replicates each).
|
| |
|
| Contributor(s) |
Sissons JR, Rothchild AC, Aderem A |
| Citation(s) |
27681624 |
| |
| Submission date |
Mar 30, 2016 |
| Last update date |
Dec 06, 2016 |
| Contact name |
Aderem Lab |
| E-mail(s) |
[email protected]
|
| Organization name |
Seattle Children's Research Institute
|
| Lab |
Aderem Lab
|
| Street address |
307 Westlake Avenue North, Suite 500
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13712 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [Alternative CDF: BrainArray version 14: MoEx10stv1_Mm_ENTREZG] |
|
| Samples (12)
|
| GSM2101755 |
WT BMM, Mtb 8h, rep 1, val |
| GSM2101756 |
WT BMM, Mtb 8h, rep 2, val |
| GSM2101757 |
WT BMM, Mtb 8h, rep 3, val |
| GSM2101758 |
miR-155-/- BMM, 0h, rep 1, val |
| GSM2101759 |
miR-155-/- BMM, 0h, rep 2, val |
| GSM2101760 |
miR-155-/- BMM, 0h, rep 3, val |
| GSM2101761 |
miR-155-/- BMM, Mtb 8h, rep 1, val |
| GSM2101762 |
miR-155-/- BMM, Mtb 8h, rep 2, val |
| GSM2101763 |
miR-155-/- BMM, Mtb 8h, rep 3, val |
|
| This SubSeries is part of SuperSeries: |
| GSE79733 |
Gene expression data from Mycobacterium tuberculosis-infected WT and miR-155-/- bone-marrow derived macrophages |
|
| Relations |
| BioProject |
PRJNA316924 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79732_RAW.tar |
317.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...