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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 05, 2016 |
| Title |
Function of deubiquitinase USP21 in mouse embryonic stem cell [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Nanog is a master pluripotency factor of embryonic stem cells (ESCs). Stable expression of Nanog is required to maintain the stemness of ESCs, although Nanog is a short-lived protein and quickly degraded by the ubiquitin-dependent proteasome system (UPS). Here, we report that the deubiquitinase USP21 interacts with, deubiquitinates and stabilizes Nanog and therefore maintains the protein level of Nanog in mouse-ESCs (mESCs). Loss of USP21 results in Nanog destruction, mESCs differentiation and reduced the somatic cell reprogramming efficiency. USP21 is a transcriptional target of the LIF/STAT3 pathway and is downregulated upon differentiation. Moreover, differentiation cues promote ERK-mediated phosphorylation and dissociation of USP21 from Nanog, thus leading to Nanog degradation. Additionally, USP21 is recruited to gene promoters by Nanog to deubiquitinate histone H2A at K119 and thus facilitates Nanog-mediated gene expression. Together, our findings provide a regulatory mechanism by which extrinsic signals regulate mESC fate via deubiquitinating Nanog.
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| Overall design |
Examination of the function of USP21 in mouse embryonic stem cells E14 cell line.
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| Contributor(s) |
Wang P, Xie X, Jiang C |
| Citation(s) |
27886188 |
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| Submission date |
Apr 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Liu Zhenping |
| E-mail(s) |
[email protected]
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| Organization name |
Tongji University
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| Street address |
1239 Si Ping Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE79891 |
Function of deubiquitinase USP21 in mouse embryonic stem cell |
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| Relations |
| BioProject |
PRJNA317333 |
| SRA |
SRP072840 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79890_RAW.tar |
60.0 Kb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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