|
| Status |
Public on Jun 20, 2007 |
| Title |
profiling of the pgpr induced systemic resistance (isr)-Arabidopsis thaliana transcriptome analysis in respons... |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
ra04-07_pgpr - profiling of the pgpr induced systemic resistance (isr) - Experiment 1 : Which genes are up- or down-regulated in Arabidopsis thaliana cultivated in vitro with increased lateral root development in response to Phyllobacterium STM196 inoculation. Experiment 2 : Which genes are up- or down-regulated during the ISR triggered by a rhizobacteria, in comparison with those affected by a pathogenic interaction. Experiment 3 : which genes are specifically induced or repressed in Arabidopsis thaliana by inoculation of the soil with a PGPR vs a bacteria that has the ability to trigger nodule formation in a Legume. - Seeds were sawn on 0.8% (W/V) agar mineral medium (see below). 4 days after storage in the dark at 4degreeC, seedling were cultivated 6 days in a growth chamber (16 h daily, 20-22degreeC) and then transferred on soil inoculated or not with 107 cfu.g-1 of Bradyrhizobium strain ORS278. Three weeks later, 3 leaves per plant were infiltrated with a suspension of Pseudomonas syringae pv. tomato (2.105 cfu.ml-1) or with MgSO4 10 mM alone for control plants. Infiltrated leaves were collected 24h later.
Keywords: normal vs rnai mutant comparaison,treated vs untreated comparison
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| |
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| Overall design |
5 dye-swap - CATMA arrays
|
| Web link |
http://urgv.evry.inra.fr/cgi-bin/projects/CATdb/consult_expce.pl?experiment_id=63
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| |
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| Contributor(s) |
Taconnat L, Renou JP, Touraine B, Martin-Magniette ML |
| Citation(s) |
18184068 |
| |
| Submission date |
Jun 01, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Véronique BRUNAUD |
| E-mail(s) |
[email protected]
|
| Organization name |
INRAE - CNRS - UPSUD
|
| Lab |
IPS2
|
| Street address |
rue Noetzlin
|
| City |
Gif-sur-Yvette |
| ZIP/Postal code |
91190 |
| Country |
France |
| |
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| Platforms (1) |
| GPL5337 |
URGV Arabidopsis thaliana 25K CATMA_v2.1 |
|
| Samples (10)
|
| GSM197546 |
12949660 - Control ISR vs Bradyrhizobium P. syringae ISR |
| GSM197547 |
12961304 - Bradyrhizobium P. syringae ISR vs Control ISR |
| GSM197548 |
12961281 - Control ISR vs Bradyrhizobium ISR |
| GSM197549 |
12961282 - Bradyrhizobium ISR vs Control ISR |
| GSM197550 |
12961284 - Pseudomonas syringae ISR vs Bradyrhizobium P. syringae ISR |
| GSM197551 |
12961287 - Bradyrhizobium P. syringae ISR vs Pseudomonas syringae ISR |
| GSM197552 |
12961290 - Bradyrhizobium P. syringae ISR vs Bradyrhizobium ISR |
| GSM197553 |
12961293 - Bradyrhizobium ISR vs Bradyrhizobium P. syringae ISR |
| GSM197554 |
12961306 - Control ISR vs Pseudomonas syringae ISR |
| GSM197555 |
12961302 - Pseudomonas syringae ISR vs Control ISR |
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| Relations |
| BioProject |
PRJNA100767 |
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