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| Status |
Public on Apr 06, 2016 |
| Title |
Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Selenium (Se) is an important trace element for many organisms and is incorporated into selenoproteins as selenocysteine (Sec). In eukaryotes, selenophosphate synthetase SPS2 is essential for Sec biosynthesis. In recent years, genetic disruptions of both Sec biosynthesis genes and selenoprotein genes have been investigated in different animal models, which provide important clues for understanding the Se metabolism and function in these organisms. However, a systematic study on the knockdown of SPS2 has not been performed in vivo.
Herein, we conducted microarray experiments to study the transcriptome of fruit flies with knockdown of SPS2 in larval and adult stages. Several hundred differentially expressed genes were identified in each stage.
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| Overall design |
Genes expression profiles of 12 fruit fly samples corresponding to larval and adult stages of SPS2 knockdown and control groups (3 replicates for each group) were analyzed using an Affymetrix Drosophila genome microarray.
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| Contributor(s) |
Li G |
| Citation(s) |
26824785 |
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| Submission date |
Apr 05, 2016 |
| Last update date |
May 04, 2018 |
| Contact name |
Yan Zhang |
| E-mail(s) |
[email protected]
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| Organization name |
Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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| Street address |
294 Tai Yuan Road Shanghai, 200031 P.R.China
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (12)
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| GSM2108618 |
SPS2 knockdown in adult stage, biological rep1 |
| GSM2108619 |
SPS2 knockdown in adult stage, biological rep2 |
| GSM2108620 |
SPS2 knockdown in adult stage, biological rep3 |
| GSM2108621 |
wild type in adult stage, biological rep1 |
| GSM2108622 |
wild type in adult stage, biological rep2 |
| GSM2108623 |
wild type in adult stage, biological rep3 |
| GSM2108624 |
SPS2 knockdown in larval stage, biological rep1 |
| GSM2108625 |
SPS2 knockdown in larval stage, biological rep2 |
| GSM2108626 |
SPS2 knockdown in larval stage, biological rep3 |
| GSM2108627 |
wild type in larval stage, biological rep1 |
| GSM2108628 |
wild type in larval stage, biological rep2 |
| GSM2108629 |
wild type in larval stage, biological rep3 |
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| Relations |
| BioProject |
PRJNA317422 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79924_RAW.tar |
22.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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