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| Status |
Public on Nov 20, 2016 |
| Title |
Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos [ChIP-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin profiling of affinity-purified nuclei of neural stem cells (NSCs) and neurons from the Drosophila embryos, respectively.
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| Overall design |
Cell type-specific nucleosome and histone modification maps were generated from NSCs and neurons nuclei, which isolated from 5–7h-old Sca-Gal4/cyo;UAS-3xFLAG-blrp-mCherry-RanGap and 12–14h-old Elav-Gal4;UAS-3xFLAG-blrp-mCherry-RanGap D. melanogaster embryos using the method INTACT (Steinner et al., 2012). We determinated nucleosome occupancy in these nuclei by micrococcal nuclease sequencing (MNase-Seq). We also performed ChIP-Seq (chromatin immunoprecipitation and sequencing) for five histone modifications (H3K4me3, H3K4me1,H3K9ac, H3K27ac, and H3K27me3). Sequencing was performed on Illumina HiSeq 2000 using signle end protocol.
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| Contributor(s) |
Ye Y, Jiang C |
| Citation(s) |
27858939, 31195140 |
| |
| Submission date |
Apr 20, 2016 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Youqiong Ye |
| E-mail(s) |
[email protected]
|
| Phone |
+8613661565591
|
| Organization name |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
280 Chongqing south road
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE80458 |
Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos |
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| Relations |
| BioProject |
PRJNA318980 |
| SRA |
SRP073601 |
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