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| Status |
Public on May 27, 2016 |
| Title |
Epitope mapping of a monoclonal antibody directed against Neisserial Heparin Binding Antigen by peptide microarray |
| Platform organism |
Neisseria meningitidis serogroup B |
| Sample organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
|
| Summary |
The aim of the study was to determine the epitope targeted by 31E10/E7 mouse monoclonal antibody (mAb) and the cross-reactivity to linear peptide epitopes of 10 different Neisserial Heparin Binding Antigen (NHBA) variants. mAb 31E10/E7 was diluted at 1:200 and incubated on a custom PepStar Peptide Microarray platform printed with 560 different peptides in three independent experiments.
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| Overall design |
A panel of 560 peptides (15-mer overlapping peptides with an offset of 4 amino acids) covering the entire sequence of ten different NHBA variants (p1, p2, p3, p5, p10, p17, p18, p20, p21 and p29) were printed in triplicate on a custom PepStar Peptide Microarray. Process-control peptide (GGSGGGSDYKDDDDK) and mouse IgG were distributed in each array copy as controls. mAb 31E10/E7 was profiled for anti-mouse IgG specific analysis of reactivity to the peptide epitopes
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| Contributor(s) |
Bartolini E, Bruttini M |
| Citation(s) |
27508302 |
| |
| Submission date |
May 12, 2016 |
| Last update date |
May 12, 2017 |
| Contact name |
Erika Bartolini |
| Organization name |
GSK Vaccines
|
| Department |
AIMB
|
| Lab |
Molecular Biology
|
| Street address |
Via Fiorentina 1
|
| City |
Siena |
| State/province |
Italy |
| ZIP/Postal code |
53100 |
| Country |
Italy |
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| Platforms (1) |
| GPL21866 |
JPT PepStar™ Peptide Microarrays_Neisserial Heparin Binding Antigen (NHBA) variants |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE81379 |
Epitope mapping of a monoclonal antibody directed against Neisserial Heparin Binding Antigen |
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| Relations |
| BioProject |
PRJNA321416 |
