 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 01, 2019 |
| Title |
CSB ablation induced apoptosis is mediated by Increased Endoplasmic Reticulum Stress Response: a gene expression study |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The DNA repair protein, Cockayne syndrome group B (CSB), has been recently identified as a promising anticancer target. Suppression, by antisense technology, of this protein causes devastating effects on tumor cells viability, through a massive induction of apoptosis, while being non-toxic to non-transformed cells. To gain insights into the mechanisms underlying the pro-apoptotic effects observed after CSB ablation, global gene expression patterns were determined, to identify genes that were significantly differentially regulated as a function of CSB expression. The one-color Agilent microarray platform was used. The study revealed that response to endoplasmic reticulum stress and response to unfolded proteins were ranked top amongst the cellular processes affected by CSB suppression.
|
| |
|
| Overall design |
A total of n=16 samples were processed: 4 experimental groups, 2 time points (6 and 12 h after transfection) and 2 biological replicates for each experimental point(time+group). The 4 experimental groups (one test and three controls) are: cells incubated with antisense oligonucleotides specifically inducing CSB mRNA degradation (ASO), mock treated cells (CTRL), transfection reagents alone (OLF) and cells treated with sense oligonucleotides (SO).
|
| |
|
| Contributor(s) |
Caputo M, Balzerano A, Arisi I, D’Onofrio M, Brandi R, Bongiorni S, Brancorsini S, Frontini M, Proietti De Santis L |
| Citation(s) |
28253359 |
| |
| Submission date |
Jun 14, 2016 |
| Last update date |
Jun 03, 2019 |
| Contact name |
Ivan Arisi |
| E-mail(s) |
[email protected]
|
| Phone |
+39-06-49255230
|
| Organization name |
European Brain Research Institute
|
| Department |
Bioinformatics Facility
|
| Street address |
viale Regina Elena 295
|
| City |
Roma |
| ZIP/Postal code |
00161 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA325696 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83367_RAW.tar |
48.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...