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| Status |
Public on Feb 08, 2008 |
| Title |
MCF7 HRG stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Heregulin beta-1 (HRG) is an extracellular ligand that activates mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-OH kinase (PI3K)/Akt signaling pathways through ErbB receptors. MAPK and Akt have been shown to phosphorylate the estrogen receptor (ER) at Ser-118 and Ser-167, respectively, thereby mimicking the effects of estrogenic activity such as estrogen responsive element (ERE)-dependent transcription. In the current study, integrative analysis was performed using two tiling array platforms, comprising histone H3 lysine 9 (H3K9) acetylation and RNA mapping, together with array comparative genomic hybridization (CGH) analysis in an effort to identify HRG-regulated genes in ER-positive MCF-7 breast cancer cells. Through application of various threshold settings, 333 (326 up-regulated and 7 down-regulated) HRG-regulated genes were detected. Prediction of upstream transcription factors (TFs) and pathway analysis indicated that 21% of HRG-induced gene regulation may be controlled by the MAPK cascade, while only 0.6% of the gene expression is controlled by ERE. A comparison with previously reported estrogen (E2)-regulated gene expression data revealed that only 12 common genes were identified between the 333 HRG-regulated (3.6%) and 239 E2-regulated (5.0%) gene groups. However, with respect to enriched upstream TFs, 4 common TFs were identified in the 14 HRG-regulated (28.6%) and 13 E2-regulated (30.8%) gene groups. These results indicated that while E2 and HRG may induce common TFs, the regulatory mechanisms that govern HRG- and E2-induced gene expression differ.
Keywords: growth hormone response
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| Overall design |
MCF-7 human breast cancer cells were incubated 2hour after administration of 10nM of the growth hormones (heregulin (HRG)). Control was set as growth hormone non-treated cells.
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| Citation(s) |
18350142 |
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| Submission date |
Jul 13, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Mariko Okada |
| E-mail(s) |
[email protected]
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| Organization name |
RIKEN RCAI
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| Lab |
Laboratory for Cellular Systems Modeling
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| Street address |
W518, 1-7-22, Suehiro-cho, Tsurumi-ku
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| City |
Yokohama |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA101553 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8471_RAW.tar |
16.5 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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