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| Status |
Public on Nov 24, 2016 |
| Title |
Identification and profiling of microRNAs in two developmental stages of the model cestode parasite Mesocestoides corti |
| Organism |
Mesocestoides corti |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as metabolism and development. The particular developmental characteristics of cestode parasites highlight the importance of studying miRNA gene regulation in these organisms. Here, we performed a comprehensive analysis of miRNAs in two developmental stages of the model cestode Mesocestoides corti. Using a high-throughput sequencing approach, we found transcriptional evidence of 42 miRNA loci in tetrathyridia larvae and strobilated worms. Tetrathyridium and strobilated worm-specific miRNAs were found, as well as differentialy expressed miRNAs between these developmental stages, suggesting miRNA regulation of stage-specific features. Moreover, it was shown that uridylation is a differential mechanism of post-transcriptional modification of M. corti miRNAs. The whole set of M. corti miRNAs represent 33 unique miRNA families, and confirm the remarkable loss of conserved miRNA families within platyhelminth parasites, reflecting their relatively low morphological complexity and high adaptation to parasitism. Overall, the presented results provide a valuable platform to studies aiming to identify and characterize novel miRNA-based molecular mechanisms of post-transcriptional gene regulation in cestodes, necessary for the elucidation of developmental aspects of the complex biology of these parasites.
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| Overall design |
Small RNA libraries from tetrathyridia (TT) and strobilated worm (ST) of M. corti were sequenced using Illumina technology. For each sample type, three libraries were constructed from three independent samples in order to have biological replicates.
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| Contributor(s) |
Basika T, Macchiaroli N, Cucher M, Espínola S, Kamenetzky L, Zaha A, Rosenzvit M, Henrique F |
| Citation(s) |
27544036 |
| |
| Submission date |
Aug 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Tatiana N Basika |
| E-mail(s) |
[email protected]
|
| Phone |
5133086070
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| Organization name |
Universidade Federal do Rio Grande do Sul
|
| Department |
Centro de Biotecnologia
|
| Lab |
Laboratório de Genômica Estrutural e Funcional
|
| Street address |
Av. Bento Gonçalves 9500, Prédios 43421/43431 - Setor IV
|
| City |
Porto Alegre |
| State/province |
Rio Grande do Sul |
| ZIP/Postal code |
91501-970 |
| Country |
Brazil |
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| Platforms (1) |
| GPL22262 |
Illumina HiSeq 2000 (Mesocestoides corti) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA336032 |
| SRA |
SRP080764 |
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