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| Status |
Public on Aug 11, 2017 |
| Title |
ChIP-seq data of RedN-venus from asynchronous and swarmer populations of Caulobacter crescentus NA1000 |
| Organism |
Caulobacter vibrioides NA1000 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Goal: We have discovered and charactherized a new Nucleoid Associated Protein in Caulobacter crescentus, that we named RedN. To determine its global binding on C.crescentus chromosome we have performed a ChIP-seq using a strain with a RedN-Venus fusion expressed at gene loci and as single copy on the chromosome.
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| Overall design |
We analyzed 2 replicates for the asynchronous population and 1 replicate for the swarmer population after synchrony of RedN-venus expressed in wild type samples (Caulobacter crescentus NA1000). We also performed 1 replicate using Venus protein expressed at xylose locus as control for non-specific binding at regions of high expression, this sample was named Mock. The total DNA before Immunoprecipitation was also extracted and sequenced and used as the control for peak detection for every biological sample, this samples were called Input.
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| Contributor(s) |
Arias-Cartin R, Dobihal G, Mehta S, Jacobs-Wagner C |
| Citation(s) |
28011580 |
| |
| Submission date |
Aug 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christine Jacobs-Wagner |
| E-mail(s) |
[email protected]
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| Phone |
203-737-6778
|
| Organization name |
Yale University
|
| Department |
Molecular, Cellular and Developmental Biology
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| Lab |
Microbial Sciences Institute, Jacobs-Wagner Lab
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| Street address |
840 West Campus Drive, PO BOX 27388
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| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
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| Platforms (1) |
| GPL18276 |
Illumina HiSeq 2000 (Caulobacter crescentus NA1000) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA338232 |
| SRA |
SRP081124 |
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