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| Status |
Public on Oct 20, 2016 |
| Title |
A small molecule inhibitor of iron-sulfur cluster assembly is toxic to Staphylococcus aureus in an Sae-dependent manner |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by array
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| Summary |
VU0038882 ('882) is a small molecule compound with antistaphylococcal activity and increased activity toward the organism during anaerobic growth conditions.
The mechanism of action of '882 is hypothesized to be saeRS dependent
S. aureus Affymetrix GeneChips were used to compare the S. aureus expression properties of wild type and isogenic ΔsaeRS mutant cells in response to '882 challenge during anaerobic growth conditions.
Significant differences were observed between the expression properties of '882 treated S. aureus wild type and saeRS mutant cells in comparison to '882 vehicle (DMSO) treated cells.
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| Overall design |
S. aureus strain Newman (wild type) or an isogenic saeRA mutant (ΔsaeRS) were grown in laboratory medium to exponential phase during anaerobic growth conditions, treated with DMSO or '882 for 10 min, total bacterial RNA was isolated, labeled and applied to Affymetrix GeneChips. We sought to determine whether the transcriptional profiles of S. aureus wild type and saeRS mutant cells differed in response to '882.
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| Contributor(s) |
Dunman PM, Skaar E |
| Citation(s) |
27773628 |
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| Submission date |
Aug 09, 2016 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Paul Dunman |
| E-mail(s) |
[email protected]
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| Phone |
585-276-5700
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| Organization name |
University of Rochester
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| Department |
Microbiology and Immunology
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| Street address |
601 Elmwood Avenue
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL1339 |
[S_aureus] Affymetrix S. aureus Genome Array |
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| Samples (8)
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| GSM2266033 |
strain Newman, anaerobic growth, DMSO treated, rep 1 |
| GSM2266034 |
strain Newman, anaerobic growth, DMSO treated, rep 2 |
| GSM2266035 |
strain Newman, anaerobic growth, '882 treated, rep 1 |
| GSM2266036 |
strain Newman, anaerobic growth, '882 treated, rep 2 |
| GSM2266037 |
strain saeRS, anaerobic growth, DMSO treated, rep 1 |
| GSM2266038 |
strain saeRS, anaerobic growth, DMSO treated, rep 2 |
| GSM2266039 |
strain saeRS, anaerobic growth, '882 treated, rep 1 |
| GSM2266040 |
strain saeRS, anaerobic growth, '882 treated, rep 2 |
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| Relations |
| BioProject |
PRJNA338302 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85379_RAW.tar |
8.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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