|
| Status |
Public on Aug 04, 2007 |
| Title |
Role of the extracytoplasmic-function sigma Factor sigmaH in Mycobacterium tuberculosis global gene expression |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by array
|
| Summary |
Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (s) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cause attenuated virulence phenotypes. In this paper, we characterize a M. tuberculosis mutant lacking the ECF s factor sigma-H. This mutant was more sensitive than the wild type to heat shock and to various oxidative stresses, but did not show decreased ability to grow inside macrophages. Using quantitative reverse transcription-PCR and microarray technology, we have started to define the sigma-H regulon and its involvement in the global regulation of the response to heat shock and the thiol-specific oxidizing agent diamide. We identified 48 genes whose expression increased after exposure of M. tuberculosis to diamide; out of these, 39 were not induced in the sigH mutant, showing their direct or indirect dependence on sigma-H. Some of these genes encode proteins whose predicted function is related to thiol metabolism, such as thioredoxin, thioredoxin reductase and enzymes involved in cysteine and molybdopterine biosynthesis. Other genes under sigma-H control encode transcriptional regulators such as sigB, sigE, and sigH itself.
Keywords: comparative genome hybridization design and genetic modification design
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| Overall design |
15 samples were analyzed. The quality controls were biological replicate and technical replicate
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| Contributor(s) |
Manganelli R, Voskuil MI |
| Citation(s) |
12123450 |
| |
| Submission date |
Aug 03, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
SMD Staff |
| E-mail(s) |
[email protected]
|
| Phone |
650-498-6012
|
| URL |
http://genome-www5.stanford.edu/
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| Organization name |
Stanford Microarray Database (SMD)
|
| Department |
Stanford University, School of Medicine
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (15)
|
| GSM215378 |
Comparison between H37Rv (wild type) and sigH mutant |
| GSM215379 |
Comparison between H37Rv (wild type) and sigH mutant rep1 |
| GSM215380 |
Comparison between H37Rv (wild type) and sigH mutant rep2 |
| GSM215381 |
Exposed to Diamide 5 mM H37Rv (wild type) |
| GSM215382 |
Exposed to Diamide 5 mM H37Rv (wild type) rep1 |
| GSM215383 |
Exposed to Diamide 5 mM H37Rv (wild type) rep2 |
| GSM215384 |
Exposed to Diamide 5 mM H37Rv (wild type) rep3 |
| GSM215385 |
Exposed to Diamide 5 mM H37Rv (wild type) rep4 |
| GSM215386 |
Exposed to Diamide 5 mM H37Rv (wild type) rep5 |
| GSM215387 |
Exposed to Diamide 5 mM sigH mutant |
| GSM215388 |
Exposed to Diamide 5 mM sigH mutant rep1 |
| GSM215389 |
Exposed to Diamide 5 mM sigH mutant rep2 |
| GSM215390 |
Exposed to Diamide 5 mM sigH mutant rep3 |
| GSM215391 |
Exposed to Diamide 5 mM sigH mutant rep4 |
| GSM215392 |
Exposed to Diamide 5 mM sigH mutant rep5 |
|
| Relations |
| BioProject |
PRJNA101907 |
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