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| Status |
Public on Mar 15, 2008 |
| Title |
Genome-wide expression and location analyses of the Candida albicans Tac1p regulon |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by array
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| Summary |
A major mechanism of azole resistance in Candida albicans is the over-expression of the genes encoding the ABC transporters Cdr1p and Cdr2p. Constitutive over-expression of these efflux pumps is due to mutations in the gene encoding Tac1p, resulting in hyperactivity of this zinc cluster transcription factor. In order to identify the transcriptional targets of Tac1p, we examined four matched sets of clinical isolates representing the development of CDR1- and CDR2-mediated azole resistance, using gene expression profiling analysis. We identified 31 genes that were consistently up-regulated with CDR1 and CDR2, including TAC1 itself, as well as 12 consistently down-regulated genes. When a resistant strain deleted for TAC1 was similarly examined, the expression of almost all of these genes was returned to levels similar to those in the matched azole-susceptible isolate.
Keywords: gene expression profiling
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| Overall design |
Four clinical match isolates (azole susceptible and resistant) were grown as three independent replicates for each set to mid-log phase. A TAC1 knockout derived from resistant isolate 5674 was also grown as three independent replicates. Each resistant isolate was compared to its susceptible parent, and the TAC1 knockout was compared to the susceptible parent (5457) of the resistant isolate, 5674.
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| Contributor(s) |
Liu TT, Znaidi S, Barker KS, Xu L, Homayouni R, Saidane S, Morschhauser J, Nantel A, Raymond M, Rogers D |
| Citation(s) |
17983269, 17905926 |
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| Submission date |
Aug 08, 2007 |
| Last update date |
Apr 16, 2020 |
| Contact name |
Katherine S Barker |
| E-mail(s) |
[email protected]
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| Organization name |
University of Tennessee Health Science Center
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| Department |
Clinical Pharmacy
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| Lab |
David Rogers laboratory
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| Street address |
881 Madison Avenue, Room 305
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| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38163 |
| Country |
USA |
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| Platforms (1) |
| GPL5723 |
[CAN04a530004N] Candida albicans affymetrix array |
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| Samples (27)
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| GSM216441 |
Resistant Isolate 17, Experiment 1 |
| GSM216443 |
Resistant Isolate 17, Experiment 2 |
| GSM216445 |
Susceptible Isolate 3, Experiment 1 |
| GSM216447 |
Susceptible Isolate 3, Experiment 2 |
| GSM216449 |
Susceptible Isolate 3, Experiment 3 |
| GSM216450 |
Resistant Isolate 17, Experiment 3 |
| GSM216452 |
Susceptible Isolate C43, Experiment 1 |
| GSM216454 |
Susceptible Isolate C43, Experiment 2 |
| GSM216455 |
Susceptible Isolate C43, Experiment 3 |
| GSM216458 |
Resistant Isolate C56, Experiment 1 |
| GSM216459 |
Resistant Isolate C56, Experiment 2 |
| GSM216461 |
Resistant Isolate C56, Experiment 3 |
| GSM216470 |
Susceptible Isolate Gu2, Experiment 1 |
| GSM216472 |
Susceptible Isolate Gu2, Experiment 2 |
| GSM216473 |
Susceptible Isolate Gu2, Experiment 3 |
| GSM216475 |
Resistant Isolate Gu5, Experiment 1 |
| GSM216478 |
Resistant Isolate Gu5, Experiment 2 |
| GSM216479 |
Resistant Isolate Gu5, Experiment 3 |
| GSM216481 |
Susceptible Isolate 5457, Experiment 1 |
| GSM216483 |
Resistant Isolate 5674, Experiment 1 |
| GSM216484 |
TAC1 knockout strain SZY31, Experiment 1 |
| GSM216485 |
Susceptible Isolate 5457, Experiment 2 |
| GSM216486 |
Resistant Isolate 5674, Experiment 2 |
| GSM216487 |
TAC1 knockout strain SZY31, Experiment 2 |
| GSM216488 |
Susceptible Isolate 5457, Experiment 3 |
| GSM216489 |
Resistant Isolate 5674, Experiment 3 |
| GSM216490 |
TAC1 knockout strain SZY31, Experiment 3 |
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| Relations |
| BioProject |
PRJNA101981 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8727_RAW.tar |
70.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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