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| Status |
Public on Jun 26, 2017 |
| Title |
Comparative transcriptome analysis of Brucella suis 1330 wild-type and DregA mutant |
| Platform organism |
Brucella suis |
| Sample organism |
Brucella suis 1330 |
| Experiment type |
Expression profiling by array
|
| Summary |
Investigation of whole genome gene expression level changes in a B. suis 1330 regA mutant, compared to the wild-type strain. The two-component system RegBA of Brucella suis plays a central role in the control of respiratory systems adapted to oxygen deficiency. The mutant strain is affected in long-term persistence in vitro (this study) and in chronic infection in vivo (Abdou, E et al. 2013, Infect.Immun. 81: 2053-61). Using an original “in vitro model of persistence”, we compare large-scale transcriptome of the wild-type and ∆regA strains to identify the RegA-regulon potentially involved in the set-up of the persistence state.
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| Overall design |
six microarrays were hybridized with (ds)cDNAs prepared from total RNA recovered from three independent cultures of the wild-type and DregA B. suis strains. Each array measures the expression level of 3,271 genes from B. suis 1330 with nineteen 60-mer probes per gene, in three replicates.
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| Contributor(s) |
Abdou E, Jubier-Maurin V, Kohler S, Pantesco V, Occhialini-Cantet A |
| Citation(s) |
28573107 |
| |
| Submission date |
Sep 30, 2016 |
| Last update date |
Jun 26, 2017 |
| Contact name |
Veronique Jubier Maurin |
| E-mail(s) |
[email protected]
|
| Organization name |
CNRS
|
| Lab |
CPBS
|
| Street address |
1919 route de Mende
|
| City |
Montpellier |
| ZIP/Postal code |
34293 |
| Country |
France |
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| Platforms (1) |
| GPL22261 |
NimbleGen Brucella suis Expression Array [2006-07-27_TI204722_60mer] |
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| Samples (6)
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| GSM2333520 |
Brucella suis 1330 DregA study wild-type A |
| GSM2333521 |
Brucella suis 1330 DregA study wild-type B |
| GSM2333522 |
Brucella suis 1330 DregA study wild-type C |
| GSM2333523 |
Brucella suis 1330 DregA study mutant A |
| GSM2333524 |
Brucella suis 1330 DregA study mutant B |
| GSM2333525 |
Brucella suis 1330 DregA study mutant C |
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| Relations |
| BioProject |
PRJNA345140 |
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