|
| Status |
Public on May 29, 2017 |
| Title |
Translation initiation factor eIF4G1 preferentially binds yeast transcript leaders containing conserved oligo-uridine motifs |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
Translational control of gene expression plays essential roles in cellular stress responses and organismal development by enabling rapid, selective, and localized control of protein production. Translational regulation depends on context-dependent differences in the protein output of mRNAs, but the key mRNA features that distinguish efficiently translated mRNAs are largely unknown. Here we comprehensively determined the RNA-binding preferences of the initiation factor eIF4G to assess whether this core translation initiation factor has intrinsic sequence preferences that contribute to preferential translation of specific mRNAs.
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| |
|
| Overall design |
Ribosome footprint profiling of eIF4G depleted yeast cells
|
| |
|
| Contributor(s) |
Zinshteyn B, Rojas-Duran MF, Gilbert WV |
| Citation(s) |
28546148 |
| |
| Submission date |
Oct 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Boris Zinshteyn |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Molecular Biology and Genetics
|
| Lab |
Green
|
| Street address |
725 N Wolfe St, PCTB 714
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA345383 |
| SRA |
SRP090827 |
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