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Series GSE88966 Query DataSets for GSE88966
Status Public on Feb 12, 2017
Title Depot dependent effects of dexamethasone on gene expression in human omental and abdominal subcutaneous adipose tissues from obese women.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used microarrays to identify transcripts regulated by dexamethasone in omental (Om) and abdominal subcutaneous (Abdsc) adipose tissues of severely obese females obtained during elective surgeries.
Minced tissue was treated with Dexamethasone in organ culture for 7 days as previously described (Lee et al, Am J Physiol Endocrinol Metab. 2011 Mar;300(3):E571-80).
 
Overall design 3 samples of Abdsc cultures and Om from the same individual was cultured in the presence of 0.7 nM insulin plus 0,1,10 or 1000 nM Dexamethasone. Adipose tissue from subject A was similtaneously cultured with 200 nM cortisol. Sample A is from one subject, Sample B and C are pooled from two different subjects each.
 
Contributor(s) Fried SK, Lee M, Pickering RT
Citation(s) 28005982
Submission date Oct 20, 2016
Last update date Jun 25, 2019
Contact name Boston University Microarray and Sequencing Resource
E-mail(s) [email protected]
Organization name Boston University
Department Microarray and Sequencing Resource
Street address 72 East Concord Street, E631
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platforms (1)
GPL15034 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [HuGene10stv1_Hs_ENTREZ, Brainarray v14]
Samples (26)
GSM2356558 Subject A_omentum_0 nM dexamethasone
GSM2356559 Subject A_omentum_1 nM dexamethasone
GSM2356560 Subject A_omentum_10 nM dexamethasone
Relations
BioProject PRJNA349255

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88966_RAW.tar 113.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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