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Series GSE8922 Query DataSets for GSE8922
Status Public on Sep 01, 2007
Title Role of EIIABMan and EIIGlc in regulation of energy metabolism, biofilms, and competence in Streptococcus mutans
Organism Streptococcus mutans
Experiment type Expression profiling by array
Summary The phosphoenolpyruvate:sugar phosphotransferase system (PTS) is the major carbohydrate transport
system in oral streptococci. The mannose-PTS of Streptococcus mutans, which transports mannose and glucose,
is involved in carbon catabolite repression (CCR) and regulates the expression of known virulence genes. In
this study, we investigated the role of EIIGlc and EIIABMan in sugar metabolism, gene regulation, biofilm
formation, and competence. The results demonstrate that the inactivation of ptsG, encoding a putative EIIGlc,
did not lead to major changes in sugar metabolism or affect the phenotypes of interest. However, the loss of
EIIGlc was shown to have a significant impact on the proteome and to affect the expression of a known virulence
factor, fructan hydrolase (fruA). JAM1, a mutant strain lacking EIIABMan, had an impaired capacity to form
biofilms in the presence of glucose and displayed a decreased ability to be transformed with exogenous DNA.
Also, the lactose- and cellobiose-PTSs were positively and negatively regulated by EIIABMan, respectively.
Microarrays were used to investigate the profound phenotypic changes displayed by JAM1, revealing that
EIIABMan of S. mutans has a key regulatory role in energy metabolism, possibly by sensing the energy levels
of the cells or the carbohydrate availability and, in response, regulating the activity of transcription factors and
carbohydrate transporters.
Keywords: Biofilms growth & development, Energy Metabolism, Gene Expression Regulation, Bacterial
 
Overall design S. mutans UA159 microarrays were provided by The
Institute for Genomic Research (TIGR). The microarrays consisted of 1,948
70-mer oligonucleotides representing 1,960 open reading frames. The full 70-mer
complement is printed four times on the surface of each microarray slide. Additional
details regarding the arrays are available at TIGR's Streptococcus mutans UA159 Genome Page
A reference RNA that had been isolated from 2
liters of UA159 cells grown in BHI broth to an optical density at 600 nm of 0.5
was used in every experiment. The reference RNA was purified as above, aliquoted,
and stored at -80°C. Our experimental conditions consisted of S. mutans
UA159 and JAM1 grown in BHI broth and collected at the mid-exponential
phase of growth (optical density at 600 nm = 0.5). Six individual Cy3-
labeled cDNA samples originating from six different cultures of UA159 or JAM1
were hybridized to the arrays along with Cy5-labeled reference cDNA, generating
a total of 12 slides.
 
Contributor(s) Abranches J, Candella MM, Wen ZT, Baker HV, Burne RA
Citation(s) 16707667
Submission date Aug 30, 2007
Last update date Oct 18, 2013
Contact name Robert A Burne
E-mail(s) [email protected]
Phone 352-846-2520
Organization name University of Florida
Department Oral Biology
Lab Burne Lab
Street address 1600 SW Archer Rd. BOX 100424
City Gainesville
State/province FL
ZIP/Postal code 32610-0424
Country USA
 
Platforms (1)
GPL4340 JCVI PFGRC Streptococcus mutans 10K v2.1 array designed primarily based on strain UA159 [FULL_FEATURE_LAYOUT]
Samples (12)
GSM226166 SmutansJAM1_BHI_rep1
GSM226171 SmutansJAM1_BHI_rep2
GSM226173 SmutansJAM1_BHI_rep3
Relations
BioProject PRJNA102329

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Supplementary file Size Download File type/resource
GSE8922_RAW.tar 3.6 Mb (http)(custom) TAR (of MEV)
Processed data included within Sample table

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