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| Status |
Public on Sep 11, 2007 |
| Title |
Contrasting infection strategies in generalist and specialist wasp parasitoids of Drosophila melanogaster. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Although host-parasitoid interactions are becoming well characterized at the organismal and cellular levels, much remains to be understood of the molecular bases for the host immune response and the parasitoids’ ability to defeat this immune response. Leptopilina boulardi and L. heterotoma, two closely related, highly infectious natural parasitoids of Drosophila melanogaster, appear to use very different infection strategies at the cellular level. Here, we further characterize cellular level differences in the infection characteristics of these two wasp species using newly derived, virulent inbred strains, and then use whole genome microarrays to compare the transcriptional response of Drosophila to each. While flies attacked by the melanogaster group specialist Leptopilina boulardi (strain Lb17) up-regulate numerous genes encoding proteolytic enzymes, components of the Toll and JAK/STAT pathways, and the melanization cascade as part of a combined cellular and humoral innate immune response, flies attacked by the generalist L. heterotoma (strain Lh14) do not appear to initiate an immune transcriptional response at the time points post-infection we assayed, perhaps due to the rapid venom-mediated lysis of host hemocytes (blood cells). Thus, the specialist parasitoid appears to invoke a full-blown immune response in the host, but suppresses and/or evades downstream components of this response. Given that activation of the host immune response likely depletes the energetic resources of the host, the specialist’s infection strategy seems relatively disadvantageous. However, we uncover the mechanism for one potentially important fitness tradeoff of the generalist’s highly immune suppressive infection strategy.
Keywords: Time series of transcriptional responses against pathogens.
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| Overall design |
The parasitoid wasps L. boulardi and L. heterotoma were allowed to attack late second instar D. melanogaster larvae (72 hrs old at 22˚C) in the following manner. Nine petri dishes containing 60 fly larvae were each exposed to six experienced L. boulardi (strain Lb17) female wasps for 2 hrs, another nine plates were exposed to five L. heterotoma (strain Lh14) females, and nine control plates were left uninfected. For each of three time points post-infection (2-5 hrs, 9-12 hrs, 21-24 hrs), 40 larvae from three replicate plates were removed and frozen at -80˚C for RNA extraction and microarray analysis (3 treatments x 3 time points x 3 replicates = 27 samples total).
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| Contributor(s) |
Schlenke TA, Morales J, Govind S, Clark AG |
| Citation(s) |
17967061 |
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| Submission date |
Sep 02, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Todd A Schlenke |
| E-mail(s) |
[email protected]
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| Phone |
404-727-0817
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| Fax |
404-727-2880
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| Organization name |
Emory University
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| Department |
Department of Biology
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| Lab |
RRC room 1081
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| Street address |
1510 Clifton Road NE
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA102357 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8938_RAW.tar |
255.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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