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| Status |
Public on Apr 18, 2018 |
| Title |
Srf destabilizes cell identity (Microarray_Agilent Technologies) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Multicellular organisms consist of multiple cell types, whose identities are maintained appropriately at locations where they are reside. The identity of each cell type is primarily maintained by cell-type-specific gene expression programs, but mechanisms that suppress these programs are poorly defined. Here we show that serum response factor (Srf), a transcription factor that is activated by various extracellular stimuli, can repress cell-type-specific genes and promote cellular reprogramming to pluripotency. Manipulations that decrease β-actin monomer resulted in nuclear accumulation of Mkl1 and the activation of Srf, which downregulated cell-type-specific genes and altered epigenetics in enhancers and chromatin organization. Mice overexpressing Srf exhibited various pathologies including an ulcerative colitis-like symptom and a metaplasia-like phenotype in the pancreas. Our results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases.
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| Overall design |
Total RNAs of NPCs (over)expressing EGFP, Mkl1, Srf, shEGFP and shActb were used to analyze effects of overexpressions of Srf and Mkl1 and knockdown of Actb on changes of gene expressions.
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| Contributor(s) |
Ikeda T, Hikichi T, Watanabe A, Okita K, Masui S |
| Citation(s) |
29643333 |
| |
| Submission date |
Nov 02, 2016 |
| Last update date |
Jul 18, 2018 |
| Contact name |
Takashi Ikeda |
| E-mail(s) |
[email protected]
|
| Organization name |
Kyoto Univ.
|
| Street address |
53 Shogoin Kawahara-cho Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA352150 |
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