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| Status |
Public on Jan 03, 2017 |
| Title |
Mesenchymal Stromal Cells Induce Ex Vivo Proliferation and Erythroid Commitment of Cord Blood Haematopoietic Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A human bone marrow-derived mesenchymal stromal cell (MSCs) and cord blood-derived CD34+ stem cell co-culture system was set up in order to evaluate the proliferative and differentiative effects induced by MSCs on CD34+ stem cells, and the reciprocal influences on gene expression profiles
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| Overall design |
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) were seeded on a plastic support. Upon reaching 80% of confluence, previously isolated CD34+ cord blood-derived hematopoietic stem cells (CB-CD34+) were plated onto a MSCs layer. After 10 days of culture, cells grown in suspension were collected in plastic tubes, while adherent cells were harvested by trypsinization. Two different fractions of HSCs were obtained: the SN-fraction, representing the HSCs grown in the supernatant of the co-cultures, and the AD-fraction, representing the HSCs grown directly in contact with the stromal layer (co-coltured MSCs). Single cultures of BM-MSCs and CB-CD34+ cells served as controls, namely MSCs-alone and CB-alone
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| Contributor(s) |
Perucca S, Russo D |
| Citation(s) |
28231331 |
| |
| Submission date |
Dec 07, 2016 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Simone Perucca |
| E-mail(s) |
[email protected]
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| Phone |
+39 0303998464
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| Organization name |
University of Brescia
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| Department |
Department of Clinical and experimental sciences
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| Lab |
Chair of Hematology
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| Street address |
Viale Europa
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| City |
Brescia |
| ZIP/Postal code |
25123 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA356502 |
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