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| Status |
Public on Jan 12, 2020 |
| Title |
Identification of miR-143 mRNA-targets by AGO-RIP in the myeloid cell line K562 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MiR-143 is a miRNA commonly found to be downregulated in a variety of cancers. The goal of this study is the identifacation of miR-143 target mRNAs by next generation sequencing (NGS) in myeloid cells after overxpression of miR-143.
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| Overall design |
We lentiviraly overexpressed miR-143 in the myeloid cell line K562. After selection of positive cells we isolated RNAs associated with the RNA induced silencing complex (RISC) by immunoprecipitation of AGO2. Isolated RNA of 3 independent experiments was subjected to next generation sequencing.
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| Contributor(s) |
Hartmann J, Behre G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 12, 2017 |
| Last update date |
Jan 12, 2020 |
| Contact name |
Gerhard Behre |
| Organization name |
Leipzig University Hospital
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| Department |
Division of Hematology and Oncology
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| Street address |
Johannisallee 32A
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| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA361086 |
| SRA |
SRP096687 |
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